Materials and methods Expression of key metabolic markers was assessed by immunohistochemistry in 480 prostate samples and prostate cell lines. The levels of glycolytic metabolism were assessed using commercial colorimetric assays. The effect of the MCT inhibitor and thioridazine was evaluated on cell viability by using the Sulforhodamine B assay. Finally, ultra-structural studies were performe...

The design, synthesis and in vitro assessment of a bifunctional imaging probe for dual fluorine ((19)F) magnetic resonance spectroscopy ((19)F-MRS) and fluorescence detection is reported. Eleven copies of 3,5-bis(trifluoromethyl)phenyl and a single copy of a sulforhodamine-B were covalently attached to a closo-B12(2-)-core via suitable linkers. The (19)F-MRS and fluorescence imaging shows that,...

This study aims to assess the comparative cytotoxic activity of ethanolic extract Mikania cordata (MC), micrantha (MM) and scandens (MS) (Family: Asteraceae) using brine shrimp lethality bioassay colorimetric sulforhodamine B assay method. In SRB assay, A549 human lung carcinoma, SK-Mel-2 skin melanoma B16F1 mouse cell lines were used. LC50 for MC, MM, MS vincristine found be 29.04, 15.84, 32.3...

Two new tetrahydroprotoberberine-aporphine dimeric alkaloids, corydaturtschines A (1) and B (2), and a new aporphine derivative, ethyl glausuccinate (3), together with 13 known protoberberine (4-16) and nine known aporphine alkaloids (17-25), were isolated from the tubers of Corydalis turtschaninovii. The structures of these new compounds were determined through spectral analyses, including ext...

In the acute respiratory distress syndrome, alveolar flooding by proteinaceous edema liquid impairs gas exchange. Mechanical ventilation is used as a supportive therapy. In regions of the edematous lung, alveolar flooding is heterogeneous, and stress is concentrated in aerated alveoli. Ventilation exacerbates stress concentrations and injuriously overexpands aerated alveoli. Injury degree is pr...

We investigate the influence of the excitation spot diameter on the laser threshold of a scattering amplifying medium. Fluorescence spectra are recorded from a suspension of TiO(2) scatterers in Sulforhodamine B dye. The threshold pump intensity becomes larger by a factor of 70 if the excitation beam diameter gets close to the mean free path?. This increase is explained by use of a simple model...