To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with a rabies vaccine by the subcutaneous (SC), intramuscular (IM) or IC route, and 10-fold the 50% lethal dose of rabies virus was inoculated into the hindleg of the immunized or non-immunized mice. The antibody titer in serum was elevated and boosted by additional immunization via all routes, but highest after th...

OBJECTIVE To investigate the effect of immunization with prokaryotically expressed recombinant fusion protein of extracellular near-transmembrane domain of Tibet minipig leptin receptor (OBR) on fat deposition in SD rats. METHODS A pair of specific primers containing BamHI and HindIII restriction enzyme sites was designed to amplify the extracellular near-transmembrane domain (1705-2364 bp) o...

Escherichia coli O157:H7 is an enteric pathogen of animals and humans that can result in deadly sequelae. Cattle are asymptomatic carriers and shedders of the bacteria and serve as an important reservoir of human infection. E. coli O157:H7 colonizes the gastrointestinal tract, most frequently at the rectoanal junction mucosa in cattle. Vaccination is a potentially highly effective means of decr...

Objective: to analyze adverse events following immunization (AEFI) caused by immunization error in the state of Paraná, Brazil, from 2003 to 2013. Method: this is a descriptive, documental, retrospective, and quantitative research using secondary data from the Adverse Event Following Immunization Information System and the Immunization Program Evaluation System. We included cases confirmed an...

In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and IgG3 isotypes from Balb/c mice immunized with cytoplasmic repetitive antigen (CRA), and flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The immunization was done by subcutaneous route three times (20 days apart) and the analysis was performed 14 days after each treatment. CRA-immunized mice produced high levels of a...

-A progress report and the research plan for a new method of antitumor immunization using the intralymphatic route are presented. A total of 205 intralymphatic infusions of intact cellular vaccines have been performed in normal and tumorbearing dogs. In vitro and in vivo experiments on normal dogs demonstrated that intralymphatic immunization produces a more rapid and more intense qytotoxic cel...

Abstract Modified Vaccinia Ankara (MVA) was recently approved as a smallpox vaccine. Variola is transmitted by respiratory droplets and MVA immunization skin scarification (s.s.) protected mice far more effectively against lethal challenge with vaccinia virus (VACV) than any other route of delivery, at lower doses. Comparisons s.s. intradermal, subcutaneous, or intramuscular routes showed that ...

BACKGROUND Diabetes accelerates atherosclerosis through undefined molecular mechanisms. Hyperglycemia induces formation of advanced glycation end product (AGE)-modified low-density lipoprotein (LDL). Anti-AGE-LDL autoantibodies favor atherosclerosis (AS) progression in humans, while anti oxidized LDL immunization inhibits AS in hypercholesterolemic, non-diabetic mice. We here investigated if AG...