Genetic mutation or loss of activin/transforming growth factor-beta (TGFbeta) receptor function has been shown in human lymphoid, breast, and colorectal tumors as well as Hep2B and Mv1Lu cell lines. Although activin stimulates FSHbeta biosynthesis and secretion, a large percentage of human gonadotroph tumors have previously been demonstrated to be nonresponsive to characterized activin effects....

molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in livestock. the insulin like growth factor-i (igf-i) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. the objectives of the present study were the estimate the haplotype frequencies of the igf-i gene polymorphisms i...

We have examined melanocytic cells derived directly from fresh biopsy tissue for the presence of p53 mutations. Using selective media that permits growth of melanocytes and inhibits growth of fibroblasts and keratinocytes, we established short-term, primary cultures of melanocytes from skin biopsies of common acquired nevi, dysplastic nevi, and from metastatic melanoma. Using PCR-single-strande...

In studies conducted on the relationship between ACTN3 R577X polymorphism and athletic performance, it is known that performance polygenic, but most sensitive provided by gene. Considering results, conformational difference much less (

Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled. We have developed an electrophoretic system having a pair of glass plates with an efficient temperature-controlling apparatus on each side of the ...

The growth hormone gene could be an attractive candidate gene for milk production in goats. Single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone (gGH) gene. For this purpose, genotyping of 90 Talli goat breeds was performed. Nine conformational patterns were observed in exon 4 of the gGH gene, with frequencies of 27.7% for the homozygous pattern (...

Polymorphism. Two alleles of the bovine prolactin gene were detected from single-stranded conformational polymorphism (SSCP) analysis of polymerase chain reaction (PCR) product. PCR Primer Sequences. 5' Primer ATT ATC TCT CTC ATT TCC TTT CA; 3' Primer ACT CTG CTG TCA CTG TCT GTA TT. PCR Conditions. 10 mM Tris, pH 8.3, 50 mM KC1, .01% gelatin, 1.5 mM MgC1, 100 pM deoxynucleoside triphosphate, .4...