نتایج جستجو برای: stirred tank bioreactor

تعداد نتایج: 23644  

Journal: :Cytotechnology 2014
Eutimio Gustavo Fernández Núñez Jaci Leme Letícia de Almeida Parizotto Wagner Antonio Chagas Alexandre Gonçalves de Rezende Bruno Labate Vale da Costa Daniela Cristina Ventini Monteiro Vera Lucia Lopes Boldorini Soraia Attie Calil Jorge Renato Mancini Astray Carlos Augusto Pereira Celso Pereira Caricati Aldo Tonso

This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-21 cell line adapted to single-cell suspension was cultured in Celligen withou...

2016
Thiarles Brun Jéssica E Rabuske Izelmar Todero Thiago C Almeida Jair J D Junior Gustavo Ariotti Tássia Confortin Jonas A Arnemann Raquel C Kuhn Jerson V C Guedes Marcio A Mazutti

The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring ra...

2017
Oluwadara Oluwaseun Alegbeleye Beatrice Olutoyin Opeolu Vanessa Jackson

This study was conducted to investigate the occurrence of PAH degrading microorganisms in two river systems in the Western Cape, South Africa and their ability to degrade two PAH compounds: acenaphthene and fluorene. A total of 19 bacterial isolates were obtained from the Diep and Plankenburg rivers among which four were identified as acenaphthene and fluorene degrading isolates. In simulated b...

2014
Nurashikin Suhaili Joo Shun Tan Mohd-Shamzi Mohamed Murni Halim

In the present work, the effects of dual impeller configurations comprising of Rushton turbine (RT) and Concave-bladed disk turbine (CD-6) on the oxygen transfer profile and fermentation performance of kojic acid production by Aspergillus flavus Link 44-1 was investigated. Batch cultivations of A. flavus Link 44-1 were performed in 2 L stirred tank bioreactor. The fermentations were conducted u...

2007
H. A. El Enshasy A. F. El Baz E. M. Ammar

The present work deals with the kinetics of rifamycins production and degradation by Amycolatopsis mediterranei in submerged culture using industrial medium. Cultivations were conducted in shake flasks and stirred tank bioreactor to evaluate the influence of transfer of process to higher scale on overall process kinetics and to obtain better understanding for the kinetics of rifamycins producti...

2013
Ali Karimi Farideh Golbabaei Momammad Reza Mehrnia Masoud Neghab Kazem Mohammad Ahmad Nikpey Mohammad Reza Pourmand

In this study, a miniature stirred tank bioreactor was designed for treatment of waste gas containing benzene, toluene and xylene. Oxygen mass transfer characteristics for various twin and single-impeller systems were investigated for 6 configurations in a vessel with 10 cm of inner diameter and working volume of 1.77L. Three types of impellers, namely, Rushton turbine, Pitched 4blades and Pitc...

2011
Martin Schaletzky Oscar Platas Barradas Henning Sievert Stefan Balabanov An-Ping Zeng Ralf Pörtner

Background During the chronic myelogenous leukemia (CML) the Bcr-Abl oncoprotein is produced, which leads to unregulated cell proliferation. CML is treated with one of several targeting therapies such as imatinib, (formerly STI-571 [Glivec; Novartis, Switzerland]) a selective inhibitor that blocks tyrosin kinase activity of the Bcr-Abl oncoprotein. Apart from the second generation Bcr-Abl inhib...

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