نتایج جستجو برای: step pcr method
تعداد نتایج: 1970830 فیلتر نتایج به سال:
Objectives: Detection of Yersinia enterocolitica in clinical samples is still not sensitive and fast enough. Polymerase chain reaction (PCR) offers the advantages of sensitivity, specificity, and rapidity. A two-step PCR assay to detect pathogenic Y: enterocolitica in infected tissue has been developed. Method: In the first step of the PCR assay, a general primer PCR amplification of the small ...
Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is an important disease of cultivated shrimp. Heavy infections may lead to retarded growth and unprofitable harvests. Existing PCR detection methods target the EHP small subunit ribosomal RNA (SSU rRNA) gene (SSU-PCR). However, we discovered that they can give false positive test results due to cross reactivity ...
a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory. While numerous papers and manuals discuss in detail conditions influencing the quality of PCR in general, relatively little has been published about the important experimental factors and the common difficulties...
applications such as high definition viedeo reproduction, portable computers, wireless, and multimedia demand, and ever-increasing need for ligh-frequency high-resolution and low-power analog-to-digital converters. flash, two-step flash, and pipeline convertors are fast but consume large amount of power and require large area. to overcome these problems, successive approximation converter blo...
in this thesis a calibration transfer method is used to achieve bilinearity for augmented first order kinetic data. first, the proposed method is investigated using simulated data and next the concept is applied to experimental data. the experimental data consists of spectroscopic monitoring of the first order degradation reaction of carbaryl. this component is used for control of pests in frui...
background: strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infections is difficult due to limitations of current parasitological and serological methods. this study was conducted to set up a pcr-based method for molecular diagnosis of strongyloides stercoralis infection by detection of copro-dna in stool samples. methods: a total of 782 fresh stool samples were c...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید