The scientific review presents the mechanisms of action intranuclear miRNAs, namely microRNA-mediated regulation non-coding RNA (ncRNA) transcriptome and alternative splicing. To write article, information was searched using Scopus, Web Science, MedLine, PubMed, Google Scholar, EMBASE, Global Health, Cochrane Library, CyberLeninka databases. It is emphasized that a significant effect on composi...

Grout sleeve splicing of rebar is a major technology in prefabricated buildings, and cementitious grout for (hereinafter called grout) an essential material this technology. Grout, with its excellent mechanical properties, improves the stability splicing. In study, properties were improved by introducing admixture form defoamer, influence defoamer on fluidity, loss rate wet apparent density str...

Forgery operations on video contents are nowadays within the reach of anyone, thanks to availability powerful and user-friendly editing software. Integrity verification authentication videos represent a major interest in both journalism (e.g., fake news debunking) legal environments dealing with digital evidence court law). While several strategies different forensics traces have been proposed ...

objective: methylmalonic acidura (mma) is a rare autosomal recessive inborn error of metabolism. in this study we present a novel nucleotide change in the mutase (mut) gene of two unrelated iranian pedigrees and introduce the methods used for its functional analysis. materials and methods: two probands with definite diagnosis of mma and a common novel variant in the mut were included in a descr...

2 Alternative pre-mRNA Splicing 17 2.1 Alternative Splicing and its Role in Regulating Gene Activities and Generating Genetic Diversity 17 2.1.1 Different Patterns of Alternative Splicing 17 2.1.2 Alternative Splicing and Genetic Diversity 18 2.2 Mechanisms Underlying Alternative Splicing Regulation 19 2.2.1 Splicing Signals and Splicing Regulatory Elements 20 2.2.2 Trans-acting Splicing Regula...

Abstract DDX39B is a member of the DEAD box (DDX) RNA helicase family required for nearly all cellular metabolic processes. The exact role and potential molecular mechanism in progression human colorectal cancer (CRC) remain to be investigated. In present study, we demonstrate that expression higher CRC tissues than adjacent normal tissues. Gain- loss-of-function assays revealed facilitates met...

Abstract We conducted a genome-wide siRNA screen for regulators of LPS-driven TNF expression in macrophages. used splicing-independent reporter the screen, and while we identified very few core spliceosome components among strongest hits, observed clear enrichment peripheral splicing regulators. Among these, novel putative regulator, UBL5, gave strong phenotype, UBL5 shRNA-expressing cell line ...

Alu elements, the most abundant retrotransposed elements in human genome, are commonly located introns and affect alternative splicing. We previously showed that an intronic antisense element enhanced splicing a minigene model. The RNA experiment was performed using consisting of exons 9–11 ACAT1 with AluSx inserted into intron 10 pCAGGS vector. Here, we explored sequence affects downstream exo...

The human IGF-I gene has six exons, four of which are alternatively spliced. Variations in splicing involving exon 5 may occur, depending on the tissue type and hormonal environment. To study the regulation of splicing to IGF-I exon 5, we established an in vitro splicing assay, using a model pre-mRNA containing IGF-I exons 4 and 5 and part of the intervening intron. Using a series of deletion m...

Tumor-associated alterations in RNA splicing result either from mutations in splicing-regulatory elements or changes in components of the splicing machinery. This review summarizes our current understanding of the role of splicing-factor alterations in human cancers. We describe splicing-factor alterations detected in human tumors and the resulting changes in splicing, highlighting cell-type-sp...