Highly purified murine granulocyte-macrophage progenitor cells (CFU-GM) were used as target cells to assess the possible direct effects of purified preparations of recombinant murine gamma-interferon, prostaglandin E, recombinant human heavy chain (acidic) ferritin, and recombinant human tumor necrosis factor alpha (TNF-alpha) on progenitor cells in vitro. Target CFU-GM, with cloning efficienci...

Mouse marrow cells were exposed to 5-fluorouracil (FU) either in vivo or in vitro and the effects on the hematopoietic stem cell compartment were studied. The drug was highly toxic to bone marrow cells including the spleen colony-forming unit (CFU-S) population. The small population of stem cells surviving FU, however, caused a different pattern of spleen colony growth when injected into lethal...

The effects of the Friend and Rauscher leukemia viruses on the marrow and spleen colony-forming unit compartments have been studied. It was found that the two viruses produced qualitatively similar but quantitatively different #{224}(ffectsrelative to each other. Comparison of the effects of the individual virus on spleen with those on the marrow also showed striking differences. In the spleen ...

Mitogenic stimulation in vitro of mouse T lymphocytes induces the production of the hematopoietic cytokines granulocyte-macrophage colony-stimulating factor and IL-3. The present experiments showed that simple crowding of murine spleen or lymph node cells was a sufficient inducing stimulus. Crowding did not have this consequence for thymus or marrow cells or spleen cells from nu/nu or Rag-1-/- ...

We examined the expression of VCAM-1 and MAdCAM-1 after bone marrow transplantation (BMT). We also examined the influence of alpha(4)beta(7) integrin blockade on the homing of cells to the bone marrow and spleen. The expression of VCAM-1 and MAdCAM-1 by endothelial cells in the spleen and bone marrow was examined by immunoelectron microscopy using colloidal gold and was analyzed semiquantitativ...

Glucan, a potent reticuloendothelial stimulant, is a glucopyranose polysaccharide derived from zymosan. Because of glucan's potential as an immunotherapeutic agent, we performed studies in order to determine its effect on granulopoiesis and macrophage production in mice. One week after the i.p. injection of 4 mg of glucan, there was a tenfold increase in colony-forming cells in the spleen and a...

Highly purified murine granulocyte-macrophage progenitor cells (CFU-GM) were used as target cells to assess the possible direct effects of purified preparations of recombinant murine 7-intcrferon, prostaglandin E, recombinant human heavy chain (acidic) ferritin, and recombinant human tumor necrosis factor a (TNF-a) on progenitor cells in vitro. Target CFU-GM, with cloning efficiencies of up to ...

*森 和博 ,瀬 戸 昭,伊 藤洋平:京 都大学医学部微生物学教室,京 都市左京区吉田近 衛町,〒606 **中 村清一:大 阪府立公衆衛生研究所 ,大 阪市東成区中道,〒537 一 Effect of sera from carbon-treated and/or irradiated mice on the colony-formation in the X-irradiated recipient mice was studied with respect to 'colony-enhancing factor(s)' and the effect of 'RES-blockade' on these factor(s). Treatment of irradiated mice with serum from carbon-injected and irradiated mi...