Three-dimensional (3D) culturing mimics the heterogeneous cellular conditions of in vivo tumor microenvironment compared to 2D monolayer-cultured cells and 3D cultures established cancer cell lines (sphere culture) or patient-derived (organoid are frequently used for research drug screening evaluation. To establish more cost time-efficient culture methods lines, we supplemented sphere medium wi...

The concept of cancer stem cells (CSCs) provides a new paradigm for understanding cancer biology. Here we report how culture conditions affect the characteristics of primary ovarian cancer cells. Cancer cells disaggregated from ovarian serous adenocarcinoma and maintained in serum-free system culture formed sphere cells that exhibited several properties expected for CSCs. These include self-ren...

The new media situation gives fuel to increased competition between narratives. In the sphere of security this poses challenges to government strategic narratives. Scholars, drawing on findings from the Anglo-Saxon sphere, suggest that the new media activism gives rise to counter-hegemonic narratives that thrive on and through social media sites. We argue that the emergence of counter-narrative...

Cancer initiating cells (CICs) represent a unique cell population essential for the maintenance and growth of tumors. Most in vivo studies of CICs utilize human tumor xenografts in immunodeficient mice. These models provide limited information on the interaction of CICs with the host immune system and are of limited value in assessing therapies targeting CICs, especially immune-based therapies....

Previous studies have demonstrated that a small population of cancer stem cell-like cells exists in retinoblastoma. To provide a model for studying this population, we sought to establish a long-term culture from human retinoblastoma that have cancer stem cell-like properties. Fresh tumor tissue was digested and cultured in serum-free medium. Tumor spheres formed and were passaged continuously....

The article discusses the ways to counteract threats national and cultural identity of Russian citizens, preserve protect people Russia in terms improving management mechanisms for monitoring controlling progress results strategic planning field culture, implementation priority tasks state policy security policy. study analyzes concepts “all-Russian identity”, “cultural sovereignty”, “problemat...

Cancer stem cells (CSCs) have been implicated in many cancers and build up an intratumor heterogeneity bestowing multiple competences on the tumor for metastasis and resistance to anti-cancer drugs [1]. Neuroblastoma is a childhood cancer arising mostly in adrenal gland and sympathetic ganglia [2]. CSCs hypothesis in neuroblastoma recently appeared [3] and was experimentally supported by the ab...

The state, by virtue of its nature, is capable controlling and regulating all spheres society. main instrument this regulation the ability to redistribute resources between different life. Russia, in era new global challenges conditions fierce information competition, needs pay special attention cultural sphere It preservation transmission values, norms experience generations that are able ensu...