Spermidine and its derivative, hypusinated eIF5A, are essential for the growth of Saccharomyces cerevisiae. Very low concentrations of spermidine (10(-8) M) are sufficient for the growth of S. cerevisiae polyamine auxotrophs (spe1Delta, spe2Delta, and spe3Delta). Under these conditions, even though the growth rate is near normal, the internal concentration of spermidine is <0.2% of the spermidi...

Schizosaccharomyces pombe cells that cannot synthesize spermidine or spermine because of a deletion-insertion in the gene coding for S-adenosylmethionine decarboxylase (Deltaspe2) have an absolute requirement for spermidine for growth. Flow cytometry studies show that in the absence of spermidine an overall delay of the cell cycle progression occurs with some accumulation of cells in the G(1) p...

The availability of fully sequenced bacterial genomes has revealed that many species known to synthesize the polyamine spermidine lack the spermidine biosynthetic enzymes S-adenosylmethionine decarboxylase and spermidine synthase. We found that such species possess orthologues of the sym-norspermidine biosynthetic enzymes carboxynorspermidine dehydrogenase and carboxynorspermidine decarboxylase...

PURPOSE To assess the effects of spermidine on the severity of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), with a focus on optic neuritis often associated with MS and EAE. METHODS Myelin oligodendrocyte glycoprotein-induced EAE mice were administered with or without spermidine at 30 mM in drinking water for 25 days. Clinical signs of EAE were s...

Several lines of evidence are presented for the involvement of spermidine in milk protein synthesis during hormone-dependent differentiation of mouse mammary epithelium in vitro. (a) When mammary explants from midpregnant mice are cultured in the presence of insulin, hydrocortisone, and prolactin, synthesis of casein and cr-lactalbumin increases markedly during the initial 48 hours. The require...

objective: in order to investigate effects of different concentration of gibberlic acid and poly amines on germination and early growth of pishtaz cultivar under salinity stress. methods: the experiment was carried out as factorial based on completely randomized design with four replicates in laboratory of agronomy and plant breeding of agricultural department, islamic azad university, varamin-...

Mouse mammary gland has been shown to possess a transport system for spermidine, spermine, and putrescine. The uptake system for spermidine, as studied in detail on mammary explants in culture is a time-dependent, energy-requiring process which can be stimulated by insulin and prolactin. The stimulatory effect of insulin involves both enhancement of V,,, for spermidine influx and prevention of ...