OBJECTIVE To examine the relationship between sperm reactive oxygen species (ROS) production and sperm morphology in a group of infertile men and healthy fertile donors. DESIGN A prospective clinical study. SETTING Male infertility clinic, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, and the Reproductive Medicine Unit, Liverpool Women's Hospital, United K...

introduction: tegrity is the most important component for transferring genetic information sperm dna fragmentation is considered a component of male infertility. its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. in this study, we investigate the relationship between sperm parameters of motility, morphology, concentration, an...

Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software (http://rsbweb.nih.gov/ij/). We describe the plug-in's functionality, and confirm its validity for sperm head morphology analysis using fish sperm. Sper...

Objective: The aim of this study was to evaluate the effects of aging on semen quality in a population of infertile couples. Methods: A cross-sectional study of semen samples obtained from 1,500 men randomly selected from couples who attended an infertility clinic was conducted. The analyses were performed using Spearman’s correlation and Mann-Whitney tests. The age groups consisted of men ≤ 35...

Since the quality of sperm is affected by water industrial or agriculture pollution, sperm motility was measured as a sensitive and accurate bioindicator of water quality. In this study, sperm motility was measured by a computer assisted sperm analysis after 24 hours exposure of sperm to different concentration of heavy metals) Cadmium and Zinc). The results show that the sperm motility was not...

BACKGROUND There are no studies relating the apoptotic marker caspase-3 in human sperm to different degrees of abnormal sperm concentration, morphology and rapid progressive motility. METHODS Semen from 67 males with abnormal semen analyses (n = 61) and normozoospermia (n = 6) were used. In each case, sperm from the neat semen (semen fraction) and after gradient centrifugation and swim-up (sw...

introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from ...

Computer-assisted sperm morphometry analysis has improved the assessment of sperm morphology, but the results depend on the use of adequate evaluation and staining procedures of spermatozoa from individual species. In this study, the morphological module of the Sperm Class Analyzer® was used for the morphometric analysis of stallion sperm heads and midpieces. Semen samples were obtained from si...

STUDY QUESTION Are there any links between the length measurements of sperm components (head, midpiece, flagellum, total sperm length and the flagellum:head ratio) and data obtained during semen analysis? SUMMARY ANSWER Both the mean measurement and the variation in the lengths of sperm components are related to characteristics of semen. WHAT IS KNOWN ALREADY Studies in non-human species ha...