To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

This work is concerned with the development and validation of two accurate, precise and specific spectrophotometric and chromatographic methods for analysis of Phenylepherine (PE), Lidocaine (LC) and Thiomersal (TM) in their ternary mixture and in Chromoderm® solution. The developed spectrophotometric method is the mean centering of ratio spectra (MCR) spectrophotometric method that depends on ...

a simple and sensitive extractive- spectrophotometric method for thedeterrnination of trace amounts of vanadium(v) is reported. the method is based on the quantitative extraction of the anion complex of v(v) with 4-(2-pyridy1azo)-resorcinol with tetrabutylammonium perchlorate into chloroform, followed by spectrophotometric measurements at 554 nm. beer's law is obeyed over the concentration...

in this study, a high-performance liquid chromatographic method (hplc) and uv spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. urine samples were applied without any extraction. the chromatographic separat...

BACKGROUND & OBJECTIVES Simple and reliable methods to estimate drugs in pharmaceutical products are needed. In most cases, antiretroviral drug estimations are performed using a HPLC method, requiring expensive equipment and trained technicians. A relatively simple and accurate method to estimate antiretroviral drugs in pharmaceutical preparations is by spectrophotometric method, which is cheap...

Three rapid, simple and accurate analytical methods for the determination of citalopram in tablets were developed. The capillary zone electrophoresis (CZE) method was carried out using fused silica capillary with 67 mM phosphate buffer pH 7.0 as the background electrolyte, 30 kV of separation voltage and UV detection at 239 nm. The direct spectrophotometric method was performed by measuring the...

A detailed study of precipitation reaction of perchlorate ion with tetraphenylarsonium chloride is reported. Optimum conditions for quantitative precipitation of perchlorate ion were determined. A gravimetric and a spectrophotometric method for perchlorate ion are proposed. The spectrophotometric method is based upon dissolution of ( C.H.),AsClO. in acetonitrile and subsequent measurement of ab...

Present study deals with two Spectrophotometric methods, Conventional Spectrophotometric Estimation and Area under Curve Method and a chromatographic method for estimation of Cefixime by using five different hydrotropic agents. These include Ammonium acetate (6M), Potassium acetate (5M), Potassium citrate (0.5 M), Sodium citrate (1.25 M) and Urea (8M). Area under curve method was based on measu...

in the present study, a simple and sensitive extractive spectrophotometric method is described for determination of buspirone. the method is based on the reaction of buspirone and bromocresol green. the ion-pair complex was quantitatively extracted into chloroform at ph 2.3 followed by spectrophotometric determination at 415 nm. the complex was stable up to 2 days and obeyed beer's law over the...

The post-antibiotic effects of gentamicin and ciprofloxacin at 1x, 2x and 4x MIC on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 were studied using a spectrophotometric method and the classic method of viable counts on agar as a reference. Monitoring of the growth kinetics was carried out by viability counting on the plate every hour and by...