Three rapid, simple and accurate analytical methods for the determination of citalopram in tablets were developed. The capillary zone electrophoresis (CZE) method was carried out using fused silica capillary with 67 mM phosphate buffer pH 7.0 as the background electrolyte, 30 kV of separation voltage and UV detection at 239 nm. The direct spectrophotometric method was performed by measuring the...

Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through hydrolysis reaction, making it interesting subject to study activity of proteases. In this work, we compared detection chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods determined limit (LOD), 0.15 ± 0.01 nM for spectropho...

in this study, a high-performance liquid chromatographic method (hplc) and uv spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. urine samples were applied without any extraction. the chromatographic separat...

Bacteria used in self-healing of concrete crack need to be embedded into the concrete in the form of dormant spores. However, quantification of viable spores is still a challengeable task due to the lack of rapid, efficient and accurate methods. Considering that viable spores have a higher refractive index than vegetative cells and the refractivity issubject to decrease during the germination p...

The aim of this work was to develop a selective cloud point extraction method for the separation and preconcentration of copper(II) prior to spectrophotometric determination. For this purpose dithizone was used as a complexing agent and the experimental solution was acidified with sulfuric acid. Triton X-114 was used as a surfactant and after phase separation, based on the cloud point of th...

A simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. The method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. The reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. The calibration graph was linear in the range of...

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 μg/ml. The relative standard devi...

In this study, derivative UV spectrophotometric methods were developed for the analysis of diflunisal, which is used as a nonsteroidal anti-inflammatory drug. Analysis wavelengths of diflunisal for the first and second order derivative UV spectrophotometric methods were obtained as 235.6 nm and 228.8 nm, respectively. The linearity ranges were found as 1.0-25.0 μg mL-1 for the first order and 1...

Micrococcus luteus was employed for the assay of rifampicin in fixed-dose combinations (FDCs) of anti-tubercular drugs containing rifampicin, isoniazid, pyrazinamide and ethambutal. The microbiological assay was compared with spectrophotometric assay for estimation of rifampicin in the FDCs as well as in dissolution studies of the FDCs. The microbiological method was found to be more sensitive ...

A highly selective methacrylic based molecularly imprinted polymer (MIP) was synthesized and applied for the separation and the pre-concentration of salbutamol in urine samples. Spectrophotometric determination of salbutamol was achieved using 2,6-dichloroquinone chlorimide as colorimetric reagent. The detection limit of the method was ca. 13 ng mL in urine after pre-concentration of the sample...