The Phadebact Pneumococcus Test is a coagglutination slide test for the serological identification of Streptococcus pneumoniae. Of 200 alpha-hemolytic streptococcal isolates, coagglutination test results agreed with those of optochin susceptibility and bile solubility in 189 cases, 105 of which were identified as S. pneumoniae by all three methods. The Phadebact test was 100% (113 of 113) sensi...

Specific diagnosis of Haemophilus pleuropneumoniae infection by the conventional culture and identification method usually requires 3 to 4 days. Since H. pleuropneumoniae may be disseminated from infected individuals during this period, this amount of time required for diagnosis may be too slow to aid in epidemic control. To obtain an earlier diagnosis, a coagglutination test was successfully u...

A coagglutination system has been devised for typing heat-stable and heat-labile antigens of Campylobacter jejuni and C. coli. The use of protein A-positive Staphylococcus aureus cells carrying Campylobacter sp. serotype antibody and the treatment of Campylobacter sp. cells with DNase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as s...

A staphylococcal coagglutination test was developed for the rapid detection of infectious hematopoietic necrosis virus (IHNV) in cell cultures and infected fish. The test could be completed in 15 min but required a minimum IHNV titer of 10(6) PFU/ml to obtain a positive reaction. All IHNV isolates, representing the five electropherotypes taken from a wide variety of species and different geogra...

A rapid technique for the immunological identification of group B streptococci in vaginal swabs is reported. Vaginal swabs obtained from 567 pregnant women at term or during labor were incubated for eight hrs in Todd Hewitt broth containing 15 micrograms per ml nalidixic acid, one microgram per ml polymixin, and 0.1 microgram per ml crystal violet (NPC broth). After streaking the swabs on blood...

Salmonella typhi antigens D, Vi and d were readily detected, by slide coagglutination, in mannitol selenite (MSB) and dulcitol selenite (DSB), Salmonella enrichment broths 4 hours after inoculation with feces from 60 patients with bacteriologically confirmed typhoid fever. Positive coagglutination also occurred using MSB and DSB inoculated with fecal specimens obtained from 16 patients from who...

This study concerns the preparation of reagents for identifying the somatic O antigens of Salmonella enteritidis. Coagglutination reagents (COAGs) with antibody fixed to killed and stabilized protein A-bearing staphylococci were prepared with antisera which were used for identifying the somatic O antigens of S. enteritidis by the slide agglutination test. The reactions of the COAGs were compare...

A coagglutination test for the identification and serotyping of Haemophilus pleuropneumoniae is described. A total of 360 H. pleuropneumoniae strains were isolated from pulmonary tissues of feeder pigs which died of acute pleuropneumonia. All of the isolates were serotyped by coagglutination, and the results were confirmed by the ring precipitation test. The most common serotype isolated in Que...

Sputum specimens from 480 patients were examined for the presence of pneumococci by Gram film and culture and for pneumococcal antigen by counterimmunoelectrophoresis, coagglutination, and latex agglutination. Ninety six positive specimens were detected. Gram film and culture provided the most reliable techniques in well taken specimens collected early in the illness before antibiotic treatment...

Sputum specimens from 480 patients were examined for the presence of pneumococci by Gram film and culture and for pneumococcal antigen by counterimmunoelectrophoresis, coagglutination, and latex agglutination. Ninety six positive specimens were detected. Gram film and culture provided the most reliable techniques in well taken specimens collected early in the illness before antibiotic treatment...