Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 ...

Sox3/SOX3 [SRY (sex determining region Y)-box 3] is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. We have previously reported characterization of the SOX3 promoter and demonstrated that the general transcription factors NF-Y (nuclear factor-Y), Sp1 (specificity protein 1) and USF (upstream stimulatory factor) are involved in tran...

SRY-related HMG-box genes (Sox genes) constitute a large family of developmentally regulated genes involved in the decision of cell fates during development and implicated in the control of diverse developmental processes. Sox3, an X-linked member of the family, is expressed in the central nervous system (CNS) from the earliest stages of development. It is considered to be one of the earliest n...

Sox proteins belong to the superfamily of high mobility group (HMG) proteins. Sox3 is expressed predominantly in the immature neuroepithelium. Ectopic expression of Sox3 causes oncogenic transformation of chicken embryo fibroblasts (CEFs). The oncogenicity of Sox3 is correlated with nuclear localization and transcriptional regulatory activity; mutants containing deletions in the HMG box or the ...

Whole mount in situ hybridisation was used to study the embryonic expression of the mouse HMG box-containing genes Sox1, Sox2 and Sox3 between 6.5 and 9.0 days post coitum (dpc). Sox2 and Sox3 are expressed in the epiblast and extraembryonic ectoderm of the egg cylinder, becoming restricted to the prospective neural plate and chorion at the onset of gastrulation. Sox3 is upregulated in the post...

SoxB1 sub-family of transcriptional regulators are expressed in progenitor (NP) cells throughout the neuroaxis and are generally downregulated during neuronal differentiation. Gain- and loss-of-function studies indicate that Sox1, Sox2 and Sox3 are key regulators of NP differentiation and that their roles in CNS development are largely redundant. Nevertheless, mutation of each SoxB1 individuall...

thesis, absorption or flow of the Cerebral Spinal Fluid (CSF) and is a common developmental disability in humans. Although CH has a significant genetic component in mice and humans, it is poorly understood at the molecular level. The Subcommissural Organ (SCO), a gland residing at the dorsal opening of the Sylvian aqueduct, has been implicated in CSF flow maintenance. We have established BAC tr...

thesis, absorption or flow of the Cerebral Spinal Fluid (CSF) and is a common developmental disability in humans. Although CH has a significant genetic component in mice and humans, it is poorly understood at the molecular level. The Subcommissural Organ (SCO), a gland residing at the dorsal opening of the Sylvian aqueduct, has been implicated in CSF flow maintenance. We have established BAC tr...

Here, we explore whether silencing via promoter DNA methylation plays a role in neural versus neural crest cell lineage decisions. We show that DNA methyltransferase3A (DNMT3A) promotes neural crest specification by directly mediating repression of neural genes like Sox2 and Sox3. DNMT3A is expressed in the neural plate border, and its knockdown causes ectopic Sox2 and Sox3 expression at the ex...

thesis, absorption or flow of the Cerebral Spinal Fluid (CSF) and is a common developmental disability in humans. Although CH has a significant genetic component in mice and humans, it is poorly understood at the molecular level. The Subcommissural Organ (SCO), a gland residing at the dorsal opening of the Sylvian aqueduct, has been implicated in CSF flow maintenance. We have established BAC tr...