نتایج جستجو برای: somatic embryo
تعداد نتایج: 107831 فیلتر نتایج به سال:
Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a tota...
the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...
The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, where...
The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, where...
The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental...
The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30...
The developmental pathway of somatic embryogenesis in Norway spruce involves proliferation of proembryogenic masses (PEMs), PEM-to-somatic embryo transition and further development of the somatic embryos. It has previously been shown that extracellular signal molecules, including arabinogalactan proteins, lipo-chitooligosaccharides and chitinases, regulate somatic embryogenesis. The Chia4-Pa1 g...
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30...
Changes in the endogenous ascorbate redox status through genetic manipulation of cellular ascorbate levels were shown to accelerate cell proliferation during the induction phase and improve maturation of somatic embryos in Arabidopsis. Mutants defective in ascorbate biosynthesis such as vtc2-5 contained ~70 % less cellular ascorbate compared with their wild-type (WT; Columbia-0) counterparts. D...
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