An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...

BACKGROUND Bacteriologic culture remains one of the most important methods to diagnose bovine tuberculosis despite the lengthy incubation time, significant decontamination and media expense, and high biocontainment requirements. Media selection is an important determination of culture sensitivity, and the planned discontinuation of the BACTEC 460 TB culture system has challenged veterinary diag...

in this paper, a finite element model is developed for the fully hydro-mechanical analysis of hydraulic fracturing in partially saturated porous media. the model is derived from the framework of generalized biot theory. the fracture propagation is governed by a cohesive fracture model. the flow within the fracture zone is modeled by the lubrication equation. the displacement of solid phase, and...

in vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. in the present investigation, the objectives were to optimize the micropropagation of rose hybrid ‘black baccarat’ cultivar. in proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (ms, ...

An examination is presented of the growth characteristics on solid media of one strain each of Brucella abortus and B. neotomae. It was observed that, during the exponential growth phase, generation times of approximately 100 min were measured for both organisms under a variety of conditions. The logarithmic growth phase did not extend beyond 18 hr in most instances. It was noted that erythrito...

trichoderma is one of the important biocontrol agents for certain plant disease that control with different mechanisms. this fungus similar to other biocontrol agents is under the influence environmently factors. medium is one of these factors. this study was investigated effect of the medium (mol.c.b.m.) containing sugar beet molasses, corn steep liquor, baker’s yeast and malt extract as the l...

reversed-phase (ISRP) material. Proteins do not adsorb on the hydrophilic exterior surfaces and do not penetrate into the hydrophobic interior surfaces, while analytes can reach the interior surfaces and be separated. (Reproduced with permission from Perry JA (1991) The internal surface reversed phase. Concept and applications. Journal of Liquid Chromatography 13: 1047}1074.) Gelfferich F (1962...