the binding of sodium n-dodecyl sulphate (sds) to aspergillus niger catalase in various ionic strengths at ph 6.4 and a temperature of 27? and 37?c has been studied over a range of sds concentrations using equilibrium dialysis and fluorescence spectroscopy techniques. the binding data were used and interpreted in terms of theoretical models (scatchard equation and wyman binding potential). the ...

the effects of sodium n-dodecyl sulphate (sds) on the structure of histone hi has been studied by a combination of e:quilibrium dialysis, u.v. spectroscopy ; polyacrylamide gel electrophoresis, protein titration and viscometery techniques using, 2.5 mm phosphate buffer, ph 6.4. the interaction of h, and sds in contrast tomanyothel-protein-sds interactions is organized between v 40 to 70. above ...

The effects of sodium n-dodecyl sulphate (SDS) on the structure of histone HI has been studied by a combination of e:quilibrium dialysis, U.V. spectroscopy ; polyacrylamide gel electrophoresis, protein titration and viscometery techniques using, 2.5 mM phosphate buffer, pH 6.4. The interaction of H, and SDS in contrast tomanyothel-protein-SDS interactions is organized between V 40 to 70. A...

(1) We have measured the incompatible phase separation that occurs in a polyethylene glycol-sodium dextran sulphate-sodium chloride-water system and have determined a critical point. (2) We have measured the activity coefficients of sodium chloride in critical-point concentrations of polyethylene glycol and sodium dextran sulphate respectively, and the osmotic coefficient of sodium dextran sulp...

The binding of bovine testicular hyaluronidase to AH-Sepharose (1,6-diaminohexane--Sepharose) gels substituted with (1) dermatan sulphate, (2) desulphated dermatan sulphate, (3) heparin and (4) de-N/O-sulphated, re-N-acetylated heparin was investigated. Hyaluronidase was found to bind to (1) and (3), but not (2) and (4). On the basis of these observations a preparative scheme for the purificati...

Relati ve viscosities of some mineral salts viz; sodium sulphate, potassium sulphate, ammon ium sulpha te and magnes iu m sulphate, at di ffe rent concentrations have been determined in binary aqueous solutions of urea (mole-frac tions of urea, XII!""" = 0.0 10, 0.025 , 0. 040, 0.055 and 0.070) at 308. 15K and in XII!""" = 0.025 at di fferent temperatures. The data have been evaluated using l o...

the interaction of sodium n-dodecyl sulphate (sds) with histones in phosphate buffer ph 6.4 has been studied spectroscopically and by equilibrium dialysis. the enthalpies of interaction and the spectroscopic data suggest different structural stability for histones-sds interaction. the linear relation between enthaly and absorbtion was determined by the equation of m= , where m is the stability ...

Two bio salt minerals, potassium sulphate, sodium sulphate and a mixture of both bio salts at the effective concentration of 1% which were added to the mulberry leaves of silkworm B. mori L., to evaluate their effect on the differential haemocyte counts (DHC) and absolute differential haemocyte counts (ADHC). Daily changes of five haemocyte types were investigated as, prohaemocytes, plasmatocyt...

Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was...