We describe a procedure for genomic difference cloning, a method for isolating sequences present in one genomic DNA population ("tester") that is absent in another ("driver"). By subtractive hybridization, a large excess of driver is used to remove sequences common to a biotinylated tester, enriching the "target" sequences that are unique to the tester. After repeated subtractive hybridization ...

In this study, an ultrasonic nanocrystalline surface modification (UNSM) technique was applied to normal and heat-treated rails made of 60 kgK steel to enhance the wear resistance of the wheel-rail interaction. The hardness and compressive residual stress values of the untreated and UNSM-treated rails were measured by the Brinell hardness tester and X-ray diffraction technique, respectively. It...

Driving a car has become a challenge for many people despite the fact that evermore technology is built into vehicles in order to support the driver. Above all, the increasing number of in-vehicle information systems (IVIS) is a main source of driver distraction. The fragmentation of IVIS elements in the cockpit increases the attention demand and cognitive load of the driver. In this paper, we ...

A robust wheel slip controller for electric vehicles is introduced. The proposed wheel slip controller exploits the dynamics of electric traction drives and conventional hydraulic brakes for achieving maximum energy efficiency and driving safety. Due to the control of single wheel traction motors in combination with a hydraulic braking system, it can be shown, that energy recuperation and vehic...

Stabilization of a single wheel mobile robot attracted researcher attentions in robotic area. However, the budget requirements for building experimental setups capable in investigating isolated parameters and implementing others encouraged the development of new simulation methods and techniques that beat such limitations. In this work we have developed a simulation platform for testing differe...

This article presents a design process of a single-wheel robot which consists of building a theoretical model, designing a mechanical structure, simulating the design, building a prototype and testing it. It describes the control strategy for this vehicle, developed during the simulation process, and how it works for a ready built prototype. It mainly focuses on the self-stabilisation problem e...

This paper presents a novel concept of mobility that provides dynamic stability and rapid locomotion. The concept, called Gyrover, is a single-wheel, gyroscopically stabilized robot that can be considered as a single wheel actuated by a spinning flywheel attached to a two-link manipulator at the wheel bearing and drive motor. The spinning flywheel acts as a gyroscope to stabilize the robot, and...

Rodents anticipate rewarding stimuli such as daily meals, mates, and stimulant drugs. When a single meal is provided daily at a fixed time of day, an increase in activity, known as food anticipatory activity (FAA), occurs several hours before feeding time. The factors affecting the expression of FAA have not been well-studied. Understanding these factors may provide clues to the undiscovered an...