DNA polymerase is essential for replication and repair. As it only performs the 5?-3? polymerization, there are two kinds of replication. One them called strand-displacement synthesis: opens double-strand (ds) to attain 3?-5?strand (leading strand) copy this template in a continuous way, other extension copies newly separated strand (lagging discontinuous manner. The complex T7 phage an optimal...

Single-strand break repair and genetic disease. DNA single-strand break repair SSBR is critical for the survival and. The association of human genetic disorders with defects in the DNA.encounter DNA single-strand breaks or other types of lesion. An inability to respond properly to, or to repair, DNA damage leads to genetic instability. Developmental disorders, Werner syndrome WS and Bloom syndr...

molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in livestock. the insulin like growth factor-i (igf-i) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. the objectives of the present study were the estimate the haplotype frequencies of the igf-i gene polymorphisms i...

To predict alterations in single-strand DNA mobility in non-denaturing electrophoretic gels, Zuker's RNA folding program was modified. Energy files utilized by the LRNA RNA folding algorithm were modified to emulate folding of single-strand DNA. Energy files were modified to disallow G-T base pairing. Stacking energies were corrected for DNA thermodynamics. Constraints on loop nucleotide sequen...

The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand reg...

The reported genome-walking methods still suffer from some deficiencies, such as cumbersome experimental steps, short target amplicon, or deep background. Here, a simple and practical fork PCR was proposed for genome-walking. employs primer set of three random oligomers to implement walking task. In primary PCR, the low-stringency amplification cycle mediates binding places on genome, producing...

The major DNA repair pathways operate on damage in double-strand DNA because they use the intact strand as a template after damage removal. Therefore, lesions in transient single-strand stretches of chromosomal DNA are expected to be especially threatening to genome stability. To test this hypothesis, we designed systems in budding yeast that could generate many kilobases of persistent single-s...