Using the single-strand conformational polymorphism technique, we have screened 66 malignant ovarian tumors for p53 mutation in exons 5 to 8. Thirty-four of the tumors demonstrated a single-strand conformational polymorphism band shift in this region of the gene, including 6 in exon 5, 7 in exon 6, 12 in exon 7, and 10 in exon 8 (one of the tumors showed a shift for exons 7 and 8). All of the s...

ghrelin is a peptide hormone that is composed of 26 amino acids and leads to a positive energy balance and obesity through stimulation of releasing growth hormone, and increasing food intake and body weight.  the purpose of this study was to determine and evaluate the association between the ghrelin gene polymorphism and growth traits in ross and cobb broiler chickens. blood samples were collec...

MATERIALS 10x Amplification buffer Include 0.01% (w/v) gelatin in the buffer. 10x TBE electrophoresis buffer Use TBE at a working strength of 1x (89 mM Tris-borate, 2 mM EDTA) for polyacrylamide gel electrophoresis. 6x Gel-loading buffer I Formamide loading buffer Human genomic DNA to be screened for point mutations Dissolve the DNA at 10 μg/ml in TE (pH 7.6). Oligonucleotide primers, forward a...

We used single-strand conformational polymorphism (SSCP) to determine apolipoprotein E (Apo E) genotypes in 47 individuals. A 295-base-pair (bp) DNA fragment coding for amino acid residues 80-178 of the Apo E protein gave distinct patterns for the three alleles. When we used SSCP to determine the Apo E polymorphism of five individuals whose phenotyping results differed from those of genotyping,...

Single-strand conformational polymorphism and denaturing gel electrophoresis were used to screen for mutations in the protoporphyrinogen oxidase gene (PPOX) of three patients with clinically and biochemically proven variegate porphyria in order to select genomic regions for specific DNA sequence analysis. Two previously undescribed mutations were identified: PPOX1423-1426-delATCT and PPOX2272in...

background: the incidence rate of breast cancer has been dramatically increasing since the last decade in iran, and it is now one of the most common female malignant tumors. b?cell lymphoma 2 (bcl2) family is the most important regulator of apoptosis, and ?938c>a single nucleotide polymorphism (snp) of bcl2 gene promoter has been demonstrated to influence breast cancer susceptibility. in this r...

familial hypercholesterolemia (fh) is an autosomal co-dominant disorder of lipid metabolism, caused by mutations in ldl receptor gene. the penetrance of fh is almost 100%, meaning that half of the offspring of affected parents born with disease. the patients are at risk of premature coronary heart disease (chd). there is no report about the molecular basis of fh in iran. identification of mutat...

Three-hundred ninety-two adult Dermacentor variabilis were collected from six Maryland counties during the spring, summer, and fall of 2002. Infection prevalence for spotted fever group Rickettsia was 3.8%, as determined by polymerase chain reaction. Single strand conformational polymorphism (SSCP) analysis followed by sequencing indicated that all infections represented a single rickettsial ta...