Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species zootechnical interest, including sheep. This study aimed to carry out bibliometric analysis scientific papers published on application SCNT sheep during period 1997–2023. The search involved Science Citation Index Expanded and Social Sciences databases main collecti...

Insulin like growth factors (IGF-I and IGF-II) are expressed in embryos and reproductive tracts of several species including cow, sheep and swine. They are mitogenic and have endocrine, paracrine and autocrine function infusing cell division, blastocyst formation, implantation and embryo growth. Increase in embryo growth will probably result with a higher implantation rates leading to consequen...

The aim of this study was to investigate the development of pulsatile GnRH secretion by GnRH neurones in primary cultures of olfactory placodes from ovine embryos. Culture medium was collected every 10 min for 8 h to detect pulsatile secretion. In the first experiment, pulsatile secretion was studied in two different sets of cultures after 17 and 24 days in vitro. In the second experiment, a se...

Our Department of Experimental Embryology originated from The Laboratory of Embryo Biotechnology, which was organized and directed by Dr. Maria Czlonkowska until her premature death in 1991. Proving successful international transfer of frozen equine embryos and generation of an embryonic sheep-goat chimaera surviving ten years were outstanding achievements of her term. In the 1990s, we produced...

BACKGROUND Improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period. OBJECTIVE This study was carried out to evaluate the effects of various concentrations of MEM vitamins during in vitro maturation of sheep oocytes and subsequent embryo development. MATERIALS AND METHODS Sheep ovaries were collected f...

The fact that embryos can be obtained in vitro undermines the role of the oviduct. However, it has been demonstrated that when in vitro produced bovine zygotes are cultured in the oviduct of sheep, cattle or mice the embryo quality is improved compared to the embryos produced in vitro. Thus the oviduct is not simply a passive organ required only for transporting the embryo to the uterus but als...