Gastrointestinal disease other than hepatobiliary and pancreatic disorders was associated with hourly rates of urinary amylase excretion above the limits of normal for control subjects (88 IU/hour compared with 69 IU/hour). In hepatobiliary disease, excretion rates of more than 88 but usually less than 190 IU/hour were sometimes found. Whilst rates of urinary amylase excretion were not helpful ...

Serum enzyme activity was sequentially determined in 10 consecutive patients with diabetic ketoacidosis, of whom all had increased -glucuronidase activity, eight had increased amylase activity, and four had increased acid phosphatase activity. Activity of amylase and that of the two lysosomal enzymes were poorly correlated, irrespective of whether peak activities or activities of all samples we...

Measurement of serum amylase is commonly used to diagnose acute pancreatitis. Moderate hyperamylasaemia is frequently seen both in acute pancreatitis and other conditions causing acute abdominal pain such as mesenteric infarction.1 2 However, grossly elevated serum amylase levels (>five times normal) are rare in non-pancreatic causes of abdominal pain.3 We report two cases ofmesenteric infarcti...

The clearance of pancreatic and salivary amylase relative to creatinine was measured in 26 control subjects and 22 patients with chronic pancreatitis. Control values for pancreatic amylase clearance (+/- SD) were 2.64 +/- 0.86% compared with 1.54 +/- 0.95% for salivary amylase. In chronic pancreatitis, pancreatic amylase clearance ratios were significantly higher than controls (P less than 0.00...

OBJECTIVE The insulin-pancreatic acinar axis may play a major role in pancreatic function. Amylase is an exocrine enzyme that is produced by pancreatic acinar cells, and low serum amylase levels may be associated with endocrine diseases, such as metabolic syndrome and diabetes. We hypothesized that low serum amylase levels may be associated with impaired islet β cell function in type 2 diabetes...

A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...

The amylase creatinine clearance ratio (ACCR) is considered to be a more sensitive index of acute pancreatitis than the serum amylase level. Serial ACCR estimations were undertaken in 25 patients undergoing an elective cholecystectomy. Using accepted criteria, 28% of these patients developed, in the postoperative period, biochemical evidence of pancreatic gland damage, although the serum amylas...

We automated a kinetic procedure for determining amylase isoenzymes in serum and urine samples. We used 4-nitro-phenylmaltoheptaoside as substrate and a selective amylase inhibitor with the Abbott-VP bichromatic system. By use of the maximum differences between pancreatic (P) and salivary (S) amylase activities remaining after inhibition by the selective inhibitor and by use of the linear range...

Macroamylasemia is characterized by an elevated serum amylase activity. It results from the circulation of a macromolecular complex consisting of amylase molecule and a serum component, often an immunoglobulin. The present study presents marked elevation of serum amylase level in a 43-year-old male, who had been admitted to our hospital for slight abdomen ache for 4 months. After treatment foll...