spermatogonial stem cell (ssc) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. the therapeutic use of embryonic stem cells (escs) is restricted due to severe ethical and immunological concerns. therefore, we need a new pluripotent cell type. despite well-known role of germ cells in the gametogenesis, some facts apparently show th...

AIM To assess proliferative and apoptotic potential of the seminiferous epithelium cells in relation to Sertoli cell maturation in newborn rats under the influence of estradiol, follicle stimulating hormone (FSH) or both agents given together. METHODS From postnatal day (PND) 5 to 15 male rats were daily injected with 12.5 microg of 17beta-estradiol benzoate (EB) or 7.5 IU of human purified F...

We have identified a liver-regulating protein involved in cell contact-mediated regulation of Sertoli cell function by primary spermatocytes in rat testis. Liver-regulating protein was studied using monoclonal antibody L8 prepared from rat primitive biliary epithelial cells. This molecule was located in vivo at the interface of Sertoli cells and spermatocytes, and expressed in a stage-dependent...

Primary cell culture is an established and widely used technique to study Sertoli cell function in vitro. However, the relative difficulty of stably overexpressing or knocking down genes in Sertoli cell culture has limited progress in the field. In this technical report, we present a method to transduce 20 dpp rat Sertoli cell cultures with VSV-G pseudotyped lentiviral based vectors at a high r...

background: artesunate is commonly used in malaria therapy. many antimalarial drugs have been associated with male reproductive dysfunction. the effect of artesunate on male reproductive activities was studied using in–vivo and in-vitro experimental models. methods: adult male rats (n=6) were orally given artesunate (2.9 mg/kg body weight) on daily basis for five days. artesunate (2.9 mg/kg bod...

introduction: complete androgen insensitivity syndrome (previously called testicular feminization) is specified by a 46 xy karyotype and negative sex chromatin, bilateral undescended testes, female genitalia appearance, and lack of mullerian derivatives. discussion: our medline search revealed that this is the first reported case of bilateral sertoli–leydig cell tumor (slct) in androgen insensi...

One hundred one azoospermic males were subjected to fine needle aspiration cytology (FNAC) of the testis and 11 of them underwent testicular biopsy as well. In 99 cases, the aspiration was adequate for classifying into different categories, which are as follows: Sertoli cell only syndrome (46), normal spermatogenesis (27), hypospermatogenesis (10), tubular/peritubular sclerosis (10) and maturat...

Background: Novel roles for the interaction of cardiotonic steroids to Na+/K+-ATPase have been established in recent years. The aim of the present study was to investigate the intracellular signaling events downstream the action of ouabain on Na+/K+-ATPase in Sertoli cell obtained from immature rats. Treatment of Sertoli cells with ouabain (1 μM) induced a rapid and transient increase in the ex...

Thyroid hormone regulates early postnatal Sertoli cell proliferation. Transient neonatal hypothyroidism allows prolonged postnatal Sertoli cell mitogenesis and doubles adult Sertoli cell numbers, testis weight, and sperm production. The mechanism of this effect is unknown. Cell proliferation is stimulated by cyclins and cyclin-dependent kinases and inhibited by cyclin-dependent kinase inhibitor...

background: the sertoli cells in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. these cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis. given that the sertoli cells play an essential rol...