Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (seroty...

Streptococcus suis bacteria are one of the most serious health problems for pigs and an emerging zoonotic agent in humans working swine industry. S. express capsular polysaccharides (CPS) a major bacterial virulence factor that define serotypes. Oligosaccharides resembling CPS serotypes 2, 3, 9, 14 have been synthesized, glycans related to 2 9 were placed on glycan array surfaces screen blood f...

results the results of this study showed some s. pneumoniae serotypes were found in both sexes and some only in one sex invasive infections. for example, serotypes 10, 14, 18 and 22 were only in female patients with infections. conclusions the analysis of the results had suggested that serotypes 6 from lung and 19 from eye are the most abundant bacterial strains isolated from patients. the dise...

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...

Clinical use of the 7-valent pneumococcal protein conjugate (PCV7) vaccine, which includes serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F, dramatically reduced invasive pneumococcal disease (IPD); however, the effectiveness was diminished due to serotype shift. Although shift due to known serotypes was anticipated, shift by misidentified serotypes was unexpected. We describe the experience with new...

a survey was carried out in five provinces of iran (kerman, sistan and baluchestan, hormozgan, khorasan and yazd) for the presence of alfalfa mosaic virus (amv) sero-types in alfalfa during 2002 to 2003. the number of samples collected was 250, represent-ing the diversity and geographical distribution of amv in these areas. diagnosis was car-ried out using polyclonal (pabs) and monoclonal (mabs...

Australia has a universal infant pneumococcal conjugate vaccination program and until recently polysaccharide vaccine for non-Indigenous adults aged ?65 years Indigenous ?50 years. We documented the impacts of adult programs on epidemiology invasive disease (IPD) in adults. IPD notifications from National Notifiable Disease Surveillance System were analysed 2002 to 2017, grouped by age, serotyp...

We developed a multiplex PCR-based reverse line blot (mPCR/RLB) assay to identify 50 uncommon pneumococcal serotypes. In combination with a previously described mPCR/RLB assay (3), all 90 pneumococcal serotypes can be identified individually (32 serotypes) or, because of predictable cross-reactions, to within small groups of two to five related serotypes (58 serotypes), which can be distinguish...

a total of 1,497 serum and blood specimens from residents of 18 different iranian communities were examined by plaque reduction neutralization test for antibodies against four (neaples, sicilian, karimabad and salehabad) phlebotomus fever virus serotypes. neutralizing antibodies against the naples and sicilian serotypes were found in every community sampled, indicating that these viruses are wi...