Purpose: Tumor genotyping using cell-free plasmaDNA (cfDNA) has the potential to allow noninvasive assessment of tumor biology, yet many existing assays are cumbersome and vulnerable to false-positive results. We sought to determine whether droplet digital PCR (ddPCR) of cfDNAwould allow highly specific and quantitative assessment of tumor genotype. ExperimentalDesign:ddPCRassays for EGFR,KRAS,...

PURPOSE Tumor genotyping using cell-free plasma DNA (cfDNA) has the potential to allow noninvasive assessment of tumor biology, yet many existing assays are cumbersome and vulnerable to false-positive results. We sought to determine whether droplet digital PCR (ddPCR) of cfDNA would allow highly specific and quantitative assessment of tumor genotype. EXPERIMENTAL DESIGN ddPCR assays for EGFR,...

In immunosuppressed hosts, the development of multidrug resistance complicates the treatment of cytomegalovirus (CMV) infection. Improved genotypic detection of impending drug resistance may follow from recent technical advances. A severely T-cell-depleted patient with chronic lymphocytic leukemia developed CMV pneumonia and high plasma viral loads that were poorly responsive to antiviral thera...

Th to N ing a thank Nuge Ad Cow 210 ann@ Serial Position 1, 4, or 7 and included either three consecutive items (in partial recall) or all items from the cued point to the end of the list and then continuing from the beginning in a circular fashion (in whole recall). Lists were spoken or printed and were sometimes temporally grouped. Specially selected comparisons demonstrated that (1) the larg...

Studies of passive transfer of cellular resistance, as manifested by refractoriness to necrotization with virulent tubercle bacilli, have shown that immune histiocytes or immune lymphocytes were effective transferring agents; immune polymorphonuclear leucocytes and immune serum as well as comparable cells from normal animals lacked this capacity. Comparisons of immune histiocytes and immune lym...

This paper presents a new topology of inductor allowing a decrease of the conductor serial resistance. After validating the concept of conductors interleaving, technological process flow and results are presented.

Previous studies showed serial 20 d in vitro passage of MRSA strain MW2 in sublethal daptomycin (DAP) resulted in diverse perturbations in both cell membrane (CM) and cell wall (CW) characteristics, including increased CM rigidity; increased CW thickness; "gain-in-function" single nucleotide polymorphisms (SNPs) in the mprF locus (i.e., increased synthesis and translocation of lysyl-phosphatidy...

resistance of streptococcus pneumoniae against penicillin is considered to be of great importance. while low-resistant strains could be treated by penicillin, treatment of highly resistant strains is very difficult and needs broad-spectrum antibiotics. this study was performed in imam khomeini medical center, tehran, iran, from 1999 to 2001 to evaluate pneumococcal resistance against penicillin...

17 In immunosuppressed hosts, development of multi-drug resistance complicates the treatment of 18 cytomegalovirus (CMV) infection. Improved genotypic detection of impending drug resistance 19 may follow from recent technical advances. A severely T-cell depleted patient with chronic 20 lymphocytic leukemia developed CMV pneumonia and high plasma viral loads poorly 21 responsive to antiviral the...