background: cystic fibrosis (cf) is a common autosomal recessive disorder that affects many body systems and is produced by mutations in the cystic fibrosis transmembrane conductance regulator (cftr) gene. cf is also the most frequently inherited disorder in the west. the aim of this study was to detect the mutations in the cftr gene in two iranian families with cf. methods: after dna extractio...

mycoplasma synoviae expressed variable lipoprotein haemagglutinin (vlha) is believed to play a major role in pathogenesis of the disease by mediating adherence and immune evasion. the aim of this study was sequencing iranian m. synoviae isolates for the detection of nucleotide variation in the m. synoviae vlha gene. using oligonucleotide primers complementary to the single-copy conserved 5´ end...

Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. The first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. The mainfocus of the present study is to perform the similarit...

introduction: influenza is a contagious acute viral disease of the respiratory tract that causes fever, headache, muscle aches and cough. one of the unique features of influenza virus is antigenic variation in viral protein neuraminidase (na) which causes emergence of new virus variants. na is responsible for the release and spread of progeny virions. due to the continuous changes of na genes, ...

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...