recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...

in recent years, the scientists have focused on nanotecnology and its applications as the most important fields of research and development. the use of nanoparticles offers many advantages, due to their unique size and physical properties. magnetic iron oxide nanoparticles have been used in many fields, because of their unique properties such as large specific surface area and convenient separa...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...

Small interfering RNA (siRNA)-induced gene silencing shows great promise in genomic research and therapeutic applications. siRNA duplexes are typically assembled from complementary synthetic oligonucleotides. High-purity single-stranded species are required for in vivo applications. Methods for separation, characterization, and purification of short RNA strands have been developed based on reve...

Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

Proteins not only play an important role in biology, but also have large potential applications in pharmaceuticals and therapeutics, food processing, textiles and leather goods, detergents, and paper manufacturing. With the development of molecular biology technologies, various kinds of proteins can be prepared from upstream processes and from biological raw materials. However, there exist vari...

background: based on the information no research has been done on the identification and isolation of anaerobic fungi in the baloochi sheep’s rumen in the dry climate up to now. objectives: the purpose of this research was the separation and study of the appearance morphology of anaerobic fungi in the baloochi sheep’s rumen in sistan region. methods: the semi-defined medium environment was used...