The cellular and subcellular localization of endogenous nitric oxide (NO.) in leaves from young and senescent pea (Pisum sativum) plants was studied. Confocal laser scanning microscopy analysis of pea leaf sections with the fluorescent probe 4,5-diaminofluorescein diacetate revealed that endogenous NO. was mainly present in vascular tissues (xylem and phloem). Green fluorescence spots were also...

Chlorophyllases (Chlases) are housekeeping proteins in plant cells. The dephytylating enzymes can catalyze chlorophyll (Chl) to form chlorophyllide, but the distribution of Chlases cells is still an interesting debate. Previously, we showed that PmCLH2 was a nuclear-encoded gene, and protein located cytosol chloroplasts Pachira macrocarpa (Pm). In this study, antibody made used by immunogold-la...

D1 protein in the PSII reaction center is the major target of photodamage, and it exhibits the highest turnover rate among all the thylakoid proteins. In this paper, rice psf (premature senescence of flag leaves) mutant and its wild type were used to investigate the genotype-dependent alteration in PSII photo-damage and D1 protein turnover during leaf senescence and its relation to ABA accumula...

Fall colors have always been fascinating and are still a remarkably puzzling phenomenon associated with the breakdown of chlorophyll (Chl) in leaves. As discovered in recent years, nongreen bilin-type Chl catabolites are generated, which are known as the phyllobilins. Collaborative chemical-biological efforts have led to the elucidation of the key Chl-breakdown processes in senescent leaves and...

Sesarmid crabs are possibly one of the most important components of mangrove fauna because of their influence on nutrient cycling and forest structure by feeding on litterfall. Little is known about the influence of electivity on the role of crabs in mangrove forests, and how this is affected by the availability of litter items. this study investigated electivity of three northern australian se...

We examined the expression of three distinct 1-aminocyclopropane-1-carboxylic acid oxidase genes during leaf ontogeny in white clover (Trifolium repens). Significant production of ethylene occurs at the apex, in newly initiated leaves, and in senescent leaf tissue. We used a combination of reverse transcriptase-polymerase chain reaction and 3'-rapid amplification of cDNA ends to identify three ...

Hemicellulose is a major component of plant cell walls and xylan the most predominant polysaccharide. Xylan degrading enzymes integrate xylanolytic system. Xylanolytic were produced by fermentation Aspergillus niger Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type used (SmF or SSF), leaves S. argentinensis (gree...

Kandelia candel (L.) Druce is the dominant mangrove species in two nature reserves in Northern Taiwan. We characterized the nutrient quality of the leaf litter originating from K. candel and attempted to suggest the extent to which the observed decline of nutrient concentrations in senescing leaves could be attributed to the withdrawal of these nutrients into the main plant before abscission. O...

Enzyme activities of tobacco leaves were compared in detergent extracts. Highest levels of chlorogenic acid oxidase, malate-NAD oxidoreductase, and acid phosphatase were obtained from bud tissues. Peroxidase activity was least in young leaves and highest in senescent leaves yellowed with Ethrel. Peroxidase zymograms obtained by means of disc electrophoresis showed differences in isozyme composi...