OBJECTIVES Although routinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of sperma...

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5 ̊C, 25 ̊C and 41 ̊C) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil extend...

A series of sequential experiments were carried out to determine optimum diluents, cryoprotectants, equilibration time, and thawing temperature for frozen duck semen in order to set up the commercial semen cryopreservating techniques which could be applied to the conservation of genetic resources, breeding, and commercial production in domestic ducks. In experiment 1, the seven semen extenders ...

BACKGROUND Intracytoplasmic sperm injection (ICSI) allows pregnancies to be established with a single sperm, improving the chances for men with severely impaired sperm quality to cause a pregnancy. Men with leukemia typically are of reproductive age and their fertility is threatened by initially impaired semen quality and cytotoxic chemotherapy. The authors examined the feasibility of sperm cry...

BACKGROUND The influence of an accompanying person (parent, guardian or nurse) on the ability of an adolescent (post-pubescent, <20 years of age) to produce a semen sample for cryopreservation, is undetermined, as is the potential for use of urine samples to retrieve sperm in those adolescents that are unable to produce a semen sample. METHODS The records from 1991-2000 inclusive were reviewe...

Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evalua...

Semen cryopreservation has become particularly important in recent years for several reasons. Long.-term survival rates have increased: more than 90% of patients with testicular cancer now survive longer than 5 years,1 making treatment-related complications such as infertility more of an issue. Assisted reproductive techniques have also improved, and cryopreserved semen from men with cancer can...

introduction: infertility is a common phenomenon in modern societies. today, use of assisted reproductive technologies (arts) for treatment of infertility is common, and cryopreservation is one of these technologies. cryopreservation has impact on the function and percentage of fertility of human sperm. in this study, motility, viability, nitric oxide, and dna apoptosis were assessed before and...

Cryopreservation of human spermatozoa is extensively used in artificial insemination and IVF programmes. Despite various advances in cryopreservation methodology, the recovery rate of functional post-thaw spermatozoa remains mediocre, with sperm motility being significantly decreased after freezing. This aim of this study was to investigate the effects of cryopreservation on both DNA integrity ...

Cytotoxic drugs and immunosuppressive therapies are used to treat patients with nonmalignant, nontesticular systemic diseases. These therapies can permanently suppress spermatogenesis. Sperm cryopreservation before treatment theoretically could give these men the opportunity to achieve a pregnancy with a woman later in life when the couple decides to do so. However, it is not known whether pret...