To examine the role of protein kinase C (PKC) in organic anion (OA) secretion, we used epifluorescence microscopy to study steady-state transepithelial secretion of 1 microM fluorescein (FL) by isolated perfused S2 segments of rabbit renal proximal tubules. Addition of 100 nM phorbol 12-myristate 13-acetate (PMA), a known PKC activator, to the bathing medium decreased steady-state secretion of ...

Secretin stimulates ductal secretion by activation of cAMP --> PKA --> CFTR --> Cl(-)/HCO(3)(-) exchanger in cholangiocytes. We evaluated the expression of alpha(2A)-, alpha(2B)-, and alpha(2C)-adrenergic receptors in cholangiocytes and the effects of the selective alpha(2)-adrenergic agonist UK 14,304, on basal and secretin-stimulated ductal secretion. In normal rats, we evaluated the effect o...

Ethanol strongly augments secretin-stimulated, but not acetylcholine (ACh)-stimulated, fluid secretion from pancreatic duct cells. To understand its mechanism of action, we examined the effect of short-chain n-alcohols on fluid secretion and intracellular Ca(2+) concentration ([Ca(2+)](i)) in guinea pig pancreatic ducts. Fluid secretion was measured by monitoring the luminal volume of isolated ...

Secretin stimulates ductal secretion by activation of cAMP⇒PKA⇒CFTR⇒Cl/HCO3 exchanger in cholangiocytes. We evaluated the expression of α-2A, α-2B, and α-2C adrenergic receptors in cholangiocytes and the effects of the selective α-2 adrenergic agonist, UK14,304, on basal and secretin-stimulated ductal secretion. In normal rats, we evaluated the effect of UK14,304 on bile and bicarbonate secreti...

BACKGROUND Secretion removal is a key issue in patients with respiratory diseases, and is known to be most effective at vibration frequencies of ∼ 13 Hz and with the greatest amplitudes possible. The Acapella devices and the water bottle are used for secretion removal in daily clinical practice but without detailed knowledge on optimal settings. The aim of this study was to evaluate the 3 diffe...

jasminum amplexicaule buch.-ham. (oleaceae) has been commonly used in the traditional medicine in dysentery, diarrhoea and bellyache in china. in the present work, the methanol extract of jasminum amplexicaule (jme) was examined for pharmacology on human colonic epithelial cell line t84 by the short-circuit current technique. the results showed that pretreatment of t84 cells with jme produced a...

The authors have developed methodology to study the kinetics of protein synthesis and secretion in filamentous fungi. Production of cellobiohydrolase I (CBHI) by Trichoderma reesei was studied by metabolic labelling of the proteins in vivo with [35S]methionine or [14C]mannose, and subsequent analysis of the labelled proteins using two-dimensional gel electrophoresis. Analysis of the different p...

Conditions necessary to detect maximal auxin-induced H(+) secretion using a macroelectrode have been investigated using corn coleoptile segments. Auxin-induced H(+) secretion is strongly dependent upon oxygenation or aeration when the tissue to volume ratio is high. Cuticle disruption or removal is also necessary to detect substantial auxin-induced H(+) secretion. The auxin-induced decrease in ...

objective: acinetobacter baumannii (a. baumannii) has a good potential to colonize on various surfaces. as a virulence factor, adhesion to surfaces is the first step in colonization. the two-partner secretion system (tps) proteins are key factors for bacterial attachment. the purpose of this study is to identify and study the role of this family of proteins in adhesion of a. baumannii to human ...

THI lacrimal gland is innervated by the lacrimal, the facial, and the cervical sympathetic nerves; the anatomical and functional characteristics of each were briefly summarized in a previous communication (Whitwell, 1958). Overwhelming evidence points to the fact that the lacrimal nerve is not secretory in function, but is purely the afferent path ofa reflex arc. Similarly, the facial nerve is ...