There is little understanding of how anionic surfactants affect the transport carbon dots (CDs) nanoparticles in soil. In this study, mobility characteristics CDs soil columns with sodium dodecyl sulfate (SDS, a model surfactant) under various conditions were investigated. The results showed that presence SDS enhanced at pH 7.0 when Na + was main background electrolyte. increased electrostatic ...

Myosin is a hexapolypeptide constituted by four light and two heavy chains the isoforms of which segregate differently in specific stages of animal development and in different fiber types in adulthood. In mammals the Myosin Heavy Chains (MHC) are polypeptides with a molecular mass of about 200 KDa which isoforms can be identified by SDS PAGE and/or immunochemistry. A method for the purificatio...

A monoacylglycerollipase (MGL) was purified from Pseudomonas sp. LP7315 by ammonium sulfate precipitation, anion-exchange chromatography, and preparative electrophoresis. The purified enzyme was homogeneous on an SDS-polyacrylamide gel with a molecular mass of 59 kDa. Its hydrolytic activity was confirmed to be specific for monoglycerides: the enzyme did not hydrolyze diand triglycerides. MGL w...

The first pathway-specific step in the biosynthesis of the core membrane diether lipids in archaebacteria is the alkylation of the primary hydroxyl group in (S).glyc. eryl phosphate by geranylgeranyl diphosphate. The reaction is catalyzed by (S)-3-0·geranylgeranylglyceryl phosphate «S)-GGGP) synthase. The cytosolic enzyme was purified to homogeneity from the moderately thermophilic archae bacte...

We describe a novel and general, mechanism-based, method for purification of xyloglucan endotransglycosylases (XETs) from crude plant extracts. Putative isoforms, obtained by step-wise precipitation with (NH4)2SO4, were incubated with tamarind xyloglucan (approximately 1 MDa) to form stable xyloglucan-XET complexes with apparent molecular masses >500 kDa on gel-permeation chromatography (GPC). ...

A novel strain, Microbacterium sp., which can be used as a catalyst in the asymmetric reduction of simple aromatic ketones to produce chiral aromatic alcohols, was isolated from soil by microbial screening. Using acetophenone as a model substrate for asymmetric reduction, 82 % reaction yield with more than 99 % enantiomeric excess of (R)-1-phenylethanol were achieved under the optimal condition...

Glycosyl-[60]fullerenes were first used as decontaminants against ricin, a lactose recognition proteotoxin in the Ricinus communis family. A fullerene glycoconjugate carrying two lactose units was synthesized by a [3 + 2] cycloaddition reaction between C60 and the azide group in 6-azidohexyl β-lactoside per-O-acetate. A colloidal aqueous solution with brown color was prepared from deprotected b...

The purification of human C8 in milligram quantities from outdated human serum was achieved by ammonium sulfate precipitation (37.5-50% saturation) and ion exchange column chromatography employing CM-32 cellulose and QAE-Sephadex. The yield of C8 activity ranged from 2-9%, and the average purification was 1,700-fold. Fully reduced C8 was shown by SDS polyacrylamide gel electrophoresis to have t...