長年，アレルゲンの解析手法としてSDS-PAGEとWestern blottingが用いられてきた．この場合，タンパク質をできるだけ可溶化するために，SDSなどの変性剤を高濃度で添加することが多い．筆者らはこの手法を用いて，甲殻類アレルゲンの種特異的反応性の違いを検出した．ところが，別の患者血清では，この方法ではバンドが全く検出されなかった．そこでこの患者血清をdot blottingで調べたところ，生では強く反応したが，80°Cで15分加熱すると反応が失われた．そこで，生の試料をdot blotしたPVDF膜を0.1％SDSを含む電気泳動バッファー中で1時間インキュベートしたところ反応性は弱まったが，完全には反応が消失していなかった．そこで，0.1％SDSを含むSDS抽出液を用いてSDS-PAGEを行ったところ，トロポミオシンのバンドが弱く反応した．甲殻類アレルゲンのSDS変性を低...

Capillary electrophoresis (CE) was used to study the polydispersity of TX-100/SDS and C12E8/SDS micelle systems. Compared with other techniques, CE provides more specific and graphically detailed information on the compositional polydispersity of such micellar systems. Clear evidence of the coexistence of micelles rich in either nonionic or anionic component has been obtained. This coexistence ...

Small Developing States (SDS) face substantial challenges in achieving sustainable development. Many of these relate to the small size and limited diversification their economies. SDS are also among most vulnerable countries impact climate change natural disasters. Meeting development goals goes hand-in-hand with building resilience. But additional costs meet resilience objectives difficult fin...

This paper characterizes the complexes formed by a small protein, ubiquitin (UBI), and a negatively charged surfactant, sodium dodecyl sulfate (SDS), using capillary electrophoresis (CE), circular dichroism (CD), and amide hydrogen-deuterium exchange (HDX; as monitored by mass spectroscopy, MS). Capillary electrophoresis of complexes of UBI and SDS, at apparent equilibrium, at concentrations of...

Using the longitudinal growth data of 13,809 individuals in Akita Prefecture, percentage distributions their adult height (AH) standard deviation scores (SDS) relation to prepubertal SDS were obtained. The AH increased with negative and decreased positive SDS, showing that a greater amount change was associated interval from mean. proportions who achieved normal stratified by as follows: 67.1%,...

BACKGROUND Models assessing characteristics contributing to response to recombinant human growth hormone (rhGH) response rarely address growth extremes in both years 1 and 2 or examine how children track from year to year. Using National Cooperative Growth Study (NCGS) data, we determined characteristics contributing to responsiveness to rhGH and the pattern of change from years 1 to 2. PATIE...

AIMS To obtain age references for sitting height (SH), leg length (LL), and SH/H ratio in the Netherlands; to evaluate how SH standard deviation score (SDS), LL SDS, SH/H SDS, and SH/LL SDS are related to height SDS; and to study the usefulness of height corrected SH/H cut-off lines to detect Marfan syndrome and hypochondroplasia. METHODS Cross-sectional data on height and sitting height were...