Coconut (Cocos nucifera L.) is one of the important palms grown both as a homestead and plantation crop in countries and most island territories of tropical regions. Different DNA-based marker systems have been utilized to assess the extent of genetic diversity in coconut. Advances in genomics research have resulted in the development of novel gene-targeted markers. In the present study, we hav...

Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency is an inborn error of ketone body metabolism and causes episodic ketoacidosis. We report clinical and molecular analyses of 5 patients with SCOT deficiency. Patients GS07, GS13, and GS14 are homozygotes of S405P, L327P, and R468C, respectively. GS17 and GS18 are compound heterozygotes for S226N and A215V, and V404F and E273X, respectivel...

To detect genetic variations among different Simmondsia chinensis genotypes, two gene targeted markers, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) were employed in terms of their informativeness and efficiency in analyzing genetic relationships among different genotypes. A total of 15 SCoT and 17 CBDP primers detected genetic polymorphism among 39 Jojoba g...

In the present investigation 28 genotypes of soybean were analysed using 37 start codon targeted (SCoT) markers and primers produced 260 DNA fragments with an average 7.03 bands per primer. From these primers, SCoT 33 65 wos most polymorphic, where 10 polymorphic amplification products detected. The lowest number amplified (2) was detected by 8, 14, 19, 31 59. Of bands, 200 (77.27 %) 5.41 To de...

The effects of 5 factors (template DNA, Mg(2+), dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymor-phism (SCoT)-PCR system of Dactylis glomerata L., using an orthogo-nal design L16 (4(5)). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction volume contained 3.0 mM Mg(2+), 0.2 mM...

OBJECTIVE Exploitation of protective metabolic pathways within injured myocardium still remains an unclarified therapeutic target in heart disease. Moreover, while the roles of altered fatty acid and glucose metabolism in the failing heart have been explored, the influence of highly dynamic and nutritionally modifiable ketone body metabolism in the regulation of myocardial substrate utilization...

This study aimed to evaluate the range of quantitative and qualitative genetic changes in chrysanthemum plants regenerated vitro from ovaries irradiated with high-energy photons (5, 10, 15 Gy) electrons (10 Gy). The highest DNA loss (up 11%) was observed originating treated 10 Gy high energy. AMOVA revealed significant differences between populations representing different mutagenic treatments ...

BACKGROUND Knowledge about genetic diversity and relationships among germplasms could be an invaluable aid in diospyros improvement strategies. METHODS This study was designed to analyze the genetic diversity and relationship of local and natural varieties in Guangxi Zhuang Autonomous Region of China using start codon targeted polymorphism (SCoT) markers. The accessions of 95 diospyros germpl...