We designed and built a combination of a scanning tunneling microscope ~STM! and a scanning electron microscope ~SEM! which is working under ultrahigh vacuum conditions ~base pressure typically 7 • 10 mbar!. The SEM is ideally used for surveying the sample and to control the STM tip positioning, while the STM extends the resolution range into the atomic scale. The design concept allows moving t...

The scanning electron microscope (SEM) has been used as a powerful tool for providing surface information of micro and nanostructures. In recent years, SEM methods for observing wet samples under atmospheric pressure have been reported by some investigators [1-2]. With these methods, the sample space is separated by a thin transparent membrane from vacuum environment where electron beam is prop...

background of the study: colorectal cancer (crc) which is the third most diagnosed cancer throughout the world is steadily rising in the asian countries with the incidence rates mimicking the western counterparts. the pre-neoplastic nature of the transitional mucosa (tm) adjoining crc lacks conclusive evidence so far. pertaining to this, the study of the mucus layer, the functional component of...

This article traces the important milestones in the development of high-resolution, field-emission, scanning electron microscopes (SEM). Such instruments are now capable of producing images of the surfaces of biological specimens that rival, in terms of resolution and contrast, those produced by conventional transmission electron microscopy (TEM). Even though one of the first instruments to pro...

An electron microprobe is an electron microscope designed for the non-destructive x-ray microanalysis and imaging of solid materials. It is essentially a hybrid instrument combining the capabilities of both the scanning electron microscope (SEM) and an x-ray fluorescence spectrometer (XRF), with the added features of fine-spot focusing (~ 1 micrometer), optical microscope imaging, and precision...

High resolution nanoscale imaging in liquid environments is crucial for studying molecular interactions in biological and chemical systems. In particular, electron microscopy is the gold-standard tool for nanoscale imaging, but its high-vacuum requirements make application to in-liquid samples extremely challenging. Here we present a new graphene based wet cell device where high resolution scan...