نتایج جستجو برای: salt bridge

تعداد نتایج: 131320  

Journal: :Chemical communications 2013
Mike P Williamson Andrea M Hounslow Joe Ford Kyle Fowler Max Hebditch Poul Erik Hansen

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

Journal: :Biochemical and biophysical research communications 2008
Yasuko Watanabe Wakako Hiraoka Yuhei Shimoyama Motohiro Horiuchi Mikinori Kuwabara Osamu Inanami

We examined the influence of D177N (D178N in humans) mutation on the conformational stability of the S2 region of moPrP(C) with varying pHs by using the SDSL-ESR technique. The ESR spectrum of D177N at pH 7.5 was narrower than that of Y161R1, referred to as WT( *). The ESR spectrum of D177N did not change when pH in the solution decreased to pH 4.0. Our results suggested that the disappearance ...

Journal: :The Journal of Cell Biology 2006
Aleksandra Czuchra Hannelore Meyer Kyle R. Legate Cord Brakebusch Reinhard Fässler

Akey feature of integrins is their ability to regulate the affinity for ligands, a process termed integrin activation. The final step in integrin activation is talin binding to the NPXY motif of the integrin beta cytoplasmic domains. Talin binding disrupts the salt bridge between the alpha/beta tails, leading to tail separation and integrin activation. We analyzed mice in which we mutated the t...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1983
A W Lee M Karplus

A generalization of the Szabo-Karplus statistical mechanical model for hemoglobin cooperativity is formulated. The model fits the available thermodynamic and spectroscopic data with assumptions that are consistent with structural results and empirical energy function calculations. It provides a mechanism of hemoglobin cooperativity that is a generalization of the proposals of Monod, Wyman, and ...

2011
Sonia Y. Lam Rachel C. Y. Yeung Tsz-Ha Yu Kong-Hung Sze Kam-Bo Wong

BACKGROUND Thermophilic enzymes are often less active than their mesophilic homologues at low temperatures. One hypothesis to explain this observation is that the extra stabilizing interactions increase the rigidity of thermophilic enzymes and hence reduce their activity. Here we employed a thermophilic acylphosphatase from Pyrococcus horikoshii and its homologous mesophilic acylphosphatase fro...

Journal: :The Journal of biological chemistry 2012
Mingjian Shi Vadim Pedchenko Briana H Greer Wade D Van Horn Samuel A Santoro Charles R Sanders Billy G Hudson Brandt F Eichman Roy Zent Ambra Pozzi

Integrin α1β1 binding to collagen IV, which is mediated by the α1-inserted (I) domain, down-regulates collagen synthesis. When unligated, a salt bridge between Arg(287) and Glu(317) is thought to keep this domain in a low affinity conformation. Ligand binding opens the salt bridge leading to a high-affinity conformation. How modulating integrin α1β1 affinity alters collagen homeostasis is unkno...

2017
Wesley M. Botello-Smith Abdelaziz Alsamarah Payal Chatterjee Chen Xie Jerome J. Lacroix Jijun Hao Yun Luo

Type 1 Serine/Threonine Kinase Receptors (STKR1) transduce a wide spectrum of biological signals mediated by TGF-β superfamily members. The STKR1 activity is tightly controlled by their regulatory glycine-serine rich (GS) domain adjacent to the kinase domain. Despite decades of studies, it remains unknown how physiological or pathological GS domain modifications are coupled to STKR1 kinase acti...

Journal: :The Journal of biological chemistry 2007
Madhusudan Dey Chune Cao Frank Sicheri Thomas E Dever

The protein kinases PKR, GCN2, and PERK phosphorylate translation initiation factor eIF2alpha to regulate general and genespecific protein synthesis under various cellular stress conditions. Recent x-ray crystallographic structures of PKR and GCN2 revealed distinct dimeric configurations of the kinase domains. Whereas PKR kinase domains dimerized in a back-to-back and parallel orientation, the ...

Journal: :Journal of molecular biology 1982
P S Kim A Bierzynski R L Baldwin

The C-peptide of ribonuclease A (residues 1 to 13) is obtained by cyanogen bromide cleavage at Met13, which converts methionine to a mixture of homoserine lactone (giving C-peptide lactone) and homoserine carboxylate (giving C-peptide carboxylate). The helix-forming properties of C-peptide lactone have been reported. The helix is formed intramolecularl$ in aqueous solution, is stabilized at’ lo...

Journal: :Blood 2008
Yoichi Imai Eun Jeong Park Dan Peer António Peixoto Guiying Cheng Ulrich H von Andrian Christopher V Carman Motomu Shimaoka

alpha(4) integrins play a pivotal role in leukocyte migration and tissue-specific homing. The ability of integrins to bind ligand is dynamically regulated by activation-dependent conformational changes triggered in the cytoplasmic domain. An NMR solution structure defined a putative membrane-proximal salt bridge between the alpha(IIb)beta(3) integrin cytoplasmic tails, which restrains integrins...

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