AIM To investigate the effect of temperature-humidity index (THI) on the expression pattern of heat-shock protein 72 (HSP72) mRNA of Sahiwal and Karan-Fries (KF) cattle in different THIs. MATERIALS AND METHODS Five different periods of a year were selected based on combinations of Tmax/Tmin, viz., P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4: >35°C/<20°C, and P5: >35°C/>20°C. The THI...

Present study was undertaken to compare the effect of two different milking methods (hand vs. machine milking) on milking ability in Murrah buffaloes and Sahiwal cows. 30 lactating Sahiwal cows and 30 lactating Murrah Buffaloes were selected and divided into four equal groups having 15 animals each. 15 animals of each species were subjected to machine milking and 15 animals of each species were...

Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism ...

The objective of the study was to compare ranking of Sahiwal bulls selected on the basis of highest lactation milk yield of their dams with their estimated breeding values (EBVs) using an animal model. Data on 23,761 lactation milk yield records of 5,936 cows from five main Livestock Experiment Stations in Punjab province of Pakistan (1964-2004) were used for the study. At present the young A.I...

Six apparently healthy, non-lactating and non-pregnant Sahiwal cows and Murrah buffalo each above two years of age were selected to evaluate the effect of seasonal variations on hematological parameters. Blood samples were collected aseptically by jugular vein puncture during peak winter and peak summer seasons. The present investigation revealed the mean values of Hb, PCV, TEC, neutrophils, MC...

The primary aim of this study was to evaluate the performance sorted semen in dairy cattle various breeds Uttarakhand. Total 6382 inseminations were performed different districts. Different factors considered for region, dam breed, parity number, insemination season, bull breed used insemination. Sorted provided by Uttarakhand Livestock Board. and statistical analysis using R version 4.3.1 soft...

The positional candidate gene DGAT1, within QTL of BTA14, harboring a lysine to alanine substitution (K232A) is significantly associated with milk production traits. The present investigation was undertaken to estimate the frequency of the DGAT1 K232A polymorphism in Indian zebu breed, Sahiwal (B. indicus; N= 51) and crossbred, Frieswal (HF X Sahiwal; N= 126) cattle using PCR-RFLP technique. De...

The major histocompatibility complex (MHC) plays well-defined roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complex of cattle is referred to as BoLA (Bovine Lymphocyte Antigen). This genetic system is among the most polymorphic. In the present study, polymorphism of the BoLADRB3.2 locus in three Bos indicus breeds viz., Sahiwal, Rathi and Ha...

An epidemiological investigation was conducted on farms in Tororo and Soroti districts of Uganda from January to February 2000 to determine the cause of reported persistent mortality of cattle. Blood and faecal material of 98 cattle comprising of 33 Friesians, 58 Zebu and 7 Sahiwal were examined. Results revealed that seven (7.1 %) cattle had trypanosome infection, mainly due to Trypanosoma viv...

The present study was carried out to observe the effect on performance, nutrient utilization, plasma enzymes and hormones in growing Sahiwal calves fed with three levels of proteins in the form of total mixed rations (TMRs). Fifteen male Sahiwal calves (12-18 month age) were distributed into three equal groups (147.35 ± 5.46 kg) and were fed on isocaloric diets containing different protein leve...