Separate populations of microtubules (MTs) distinguishable by their level of posttranslationally modified tubulin subunits and by their stability in vivo have been described. In polarized 3T3 cells at the edge of an in vitro wound, we have found a striking preferential coalignment of vimentin intermediate filaments (IFs) with detyrosinated MTs (Glu MTs) rather than with the bulk of the MTs, whi...

Mitotic spindles of Schizosaccharomyces pombe have been studied by EM, using serial cross sections to reconstruct 12 spindles from cells that were ultrarapidly frozen and fixed by freeze substitution. The resulting distributions of microtubules (MTs) have been analyzed by computer. Short spindles contain two kinds of MTs: continuous ones that run from pole to pole and MTs that originate at one ...

We have analyzed the fine structure of 10 chromosomal fibers from mitotic spindles of PtK1 cells in metaphase and anaphase, using electron microscopy of serial thin sections and computer image processing to follow the trajectories of the component microtubules (MTs) in three dimensions. Most of the kinetochore MTs ran from their kinetochore to the vicinity of the pole, retaining a clustered arr...

The polarity of kinetochore microtubules (MTs) has been studied in lysed PtK1 cells by polymerizing hook-shapdd sheets of neurotubulin onto the walls of preexisting cellular MTs in a fashion that reveals their structural polarity . Three different approaches are presented here : (a) we have screened the polarity of all MTs in a given spindle cross section taken from the region between the kinet...

In the present research, vibrational behavior of anisotropic protein microtubules (MTs) immersed in cytosol via Cooper–Naghdi shell model is investigated. MTs are hollow cylindrical structures in the eukaryotic cytoskeleton which surrounded by filament network. The temperature effect on vibration frequency is also taken into account by assuming temperature-dependent material properties for MTs....

Tannic acid fixation reveals differences in the number of protofilaments between microtubules (MTs) in the nematode Caenorhabditis elegans. Most cells have MTs with 11 protofilaments but the six touch receptor neurons (the microtubule cells) have MTs with 15 protofilaments. No 13-protofilament (13-p) MT has been seen. The modified cilia of sensory neurons also possess unusual structures. The ci...

Geographic Information System (GIS) was used as a decision-making tool to target potential geothermal power plant sites in Sabalan geothermal field, northwestern part of Iran. The aim of the study was to identify suitable areas to establish 55 MW geothermal power plants (GPP). After comprehensive study about required data for GPP site selection project, available data in the area on a regional ...

In this study, the chemical composition of the essential oils (EOs) extracted from aerial parts of native populations of Origanum vulgare ssp. vulgare from Iran was compared by GC and GC/MS. Plants were collected at bloom stage from their natural habitats located in Kaleybar, Sabalan, Meshkin Shahr and Chalus regions, respectively from East Azerbaijan, Ardabil, Ardabil and Mazandaran provinces....