the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

background: the pathogenesis of gestational trophoblastic disease (gtd) is not clearly known. objective: in this study, immunoexpression of proteins bcl-2 and beclin-1 in trophoblastic lesions and normal trophoblastic tissue was conducted to study the mechanism of apoptotic and autophagic cell death that is expected to complete the study of gtd pathogenesis. materials and methods: bcl-2 and bec...

this study investigated the association between hsv-1 infection and atopy by comparing seropositivity to hsv-1 in atopic children with asthma and allergic rhinitis and in non-atopic children. totally 249 children randomly selected from the university outpatient pediatric clinics were prospectively enrolled in the study between september 1 and november 30, 2007. serum samples were examined using...

it is widely thought that bcl-2 family proteins regulate commitment to apoptosis primarily through their capacity to control the permeability of the mitochondrial outer membrane permeabilization (momp) triggers the release of multiple apoptogenic factors into the cytosol and, thereby apoptosis. various bcl-2 family members affect this key event of the apoptotic cascade in different ways, determ...

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

alnus glutinosa, fraxinus excelsior and papaver rhoeas have long been used in folkloric medicine for the treatment of various ailments. as part of our continuing screening of plant extracts for activities, the extracts of a. glutinosa, f. excelsior and p. rhoeas have been screened for their antioxidant and antibacterial activities, as well as their general toxicity towards brine shrimps. among ...

background :cytotoxic t lymphocyte-associated antigen-4 (ctla-4) is an important negative regulator of t-cell responses. the -1722tc polymorphism of the ctla-4 gene may be associated with systemic lupus erythematosus (sle) risk, but related results from previous studies have been inconsistent. we carried out a meta-analysis to assess this association more precisely.   methods : a systematic sea...