The establishment of a fungal population composed of the main species usually found in ruminants in the rumen of gnotobiotic lambs did not significantly alter the in sacco digestibility of meat meal and soybean cake. The proteolytic activity of the rumen fluid against 14C-casein was not affected by the fungi. Therefore, these microorganisms probably do not play an important role in the degradat...

this experiment was conducted to determine the effect of procceesing of sugarcane pith with high pressure steam (210-220c◦, at 19 bar, over 3 min, on 70 % moisture) on fermentative activity and gas production parameters and degradation by pure microorganism of rumen. the microbial different groups of rumen were separated by physical and chemical methods and using antibiotics. these microbial gr...

Rumen of cattle harbors many microorganisms responsible for bioconversion of nutrients into a source of energy for the animals. In recent years many rumen microbes have been isolated and characterized by sequence analysis of 16S ribosomal RNA gene. Some of the microbes have also been recommended as feed additives for improving the overall growth or production of animals. Rumen bacteria which ha...

Kalal!njuk G. I., M. Marounek, M. G. Gerasymiv, O. G. Savka, L. G. Kalal!njuk: Activity of Dehydrogenases and Enzymes of Nitrogen Metabolism in Rumen Mucosa and Liver of Steers Fed Monensin. Acta vet. Bmo, 62, 1993: 127-131. The effect of monensin on activity of intracellular enzymes of tissues was determined in ten steers fed hay, silage and concentrate. Five steers received monensin in amount...

Two groups of Merino sheep (with or without rumen fistulae) were used to study the effect of feeding maize-silage (compared to hay) on: thiamine concentrations in rumen fluid, tissues and organ; blood transketolase activity; microbial fermentation in the rumen. After sheep were transferred from a hay diet to maize-silage, thiamine concentrations in ruminal fluid fell to about 25% of control val...

The polymerase chain reaction (PCR) technique was used to investigate the fate of a transgene in the rumen of sheep fed silage and maize grains from an insect-resistant maize line. A 1914-bp DNA fragment containing the entire coding region of the synthetic cryIA(b) gene was still amplifiable from rumen fluid sampled 5 h after feeding maize grains. The same target sequence, however, could not be...

Twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse Ala2, Ala5, GlyArg-4-methoxy-2-naphthylamide (GlyArg-MNA) and Leu-MNA. Several species, notably Megasphaera elsdenii, were active against Ala2, and a smaller number, including Bacteroides ruminicola, Butyrivibrio fibrisolvens, Ruminococcus flavefaciens, Lachnospira multipara and Ruminobacter amyloph...

HOUPT, T. RICHARD, AND KATHERINE A. HOUPT. Transfer of urea nitro,gen across the rumen wall. Am. J. Physiol. 2 1 /t(6) : 1296-1303. 1968.-Net movement of urea N across the wall of Pavlov-type rumen pouches was measured in three goats and one sheep as urea concentration difference between pouch and blood was varied. When urease activity of the rumen epitheliurn was depressed by vigorous rinsing ...

bengal, asparagus agar (Allen, 1951), asparagus agar plus 1:80,000 crystal violet, and asparagus agar plus 1:15,000 rose bengal. The addition of dyes decreased the number of contaminants but also appeared to inhibit the development of the rhizobia. All plates were examined daily for 2 weeks. Smears of colonies which resembled rhizobia were examined. Pure cultures were obtained by the use of bot...