Recent work has shown that NMR structures can be determined by integrating sparse NMR data with structure prediction methods such as Rosetta. The experimental data serve to guide the search for the lowest energy state towards the deep minimum at the native state which is frequently missed in Rosetta de novo structure calculations. However, as the protein size increases, sampling again becomes l...

The Rosetta Stone Methodology (RSM) has been developed which allows organizations to undertake Software Process Improvement (SPI) based on businessand organizational-driven goals and objectives. The methodology itself is fully customizable and allows organizations to make adjustments to the model where they feel it appropriate. To demonstrate the usefulness, appropriateness and practicality of ...

Significant progress has recently been made in de novo protein structure prediction. The Rosetta method by Baker and colleagues, which is based on the idea of assembling putative models from a library of k-mer fragments derived from known three-dimensional protein structures, proved to be particularly successful. Critical components of the Rosetta approach are various sequence-dependent as well...

Noncanonical amino acids (NCAAs) can be used in a variety of protein design contexts. For example, they can be used in place of the canonical amino acids (CAAs) to improve the biophysical properties of peptides that target protein interfaces. We describe the incorporation of 114 NCAAs into the protein-modeling suite Rosetta. We describe our methods for building backbone dependent rotamer librar...

The international Rosetta mission was launched in 2004 and consists of the orbiter spacecraft Rosetta and the lander Philae. The aim of the mission is to map the comet 67P/Churyumov-Gerasimenko by remote sensing, and to examine its environment in situ and its evolution in the inner Solar System. Rosetta was the first spacecraft to rendezvous with and orbit a comet, accompanying it as it passes ...

Rosetta is a powerful software suite for the modeling and design of macromolecules [1]. Originally written within the laboratory of David Baker, the Rosetta developers community (RosettaCommons, https://www.rosettacommons.org/) has expanded to encompass hundreds of developers across tens of institutions. The Rosetta community, from software developers to academic and industry users, meets yearl...

The European Space Agency's Rosetta mission ended operations on 30 September 2016 having spent over 2 years in close proximity to its target comet, 67P/Churyumov-Gerasimenko. Shortly before this, in summer 2016, a discussion meeting was held to examine how the results of the mission could be framed in terms of cometary and solar system science in general. This paper provides a brief history of ...

Rosetta ab initio protein structure predictions in CASP4 were considerably more consistent and more accurate than previous ab initio structure predictions. Large segments were correctly predicted (>50 residues superimposed within an RMSD of 6.5 A) for 16 of the 21 domains under 300 residues for which models were submitted. Models with the global fold largely correct were produced for several ta...