introduction: potential almond rootstock and thus main genetic sources for the breeding of its new rootstocks are almond itself, peach and plum, and these species cross-breeds almond is a source of resistance to limestone and to drought because of its roots, which reach deep layers of soil; it is also more resistant than peach to na and bo. asexual or vegetative propagation is a hereditable cha...

Traditional plant breeding is often limited by the genetic diversity within a species. The use of biotechnology allows introducing into a plant, specific traits that come from the same or another plant species. In this paper, we focus on tissue culture of sunflower (Helianthus annuus L., Asteraceae), particularly related to rooting transgenic sunflower plantlets. Sunflower is notoriously recalc...

The two commercially important apple rootstocks i.e., MM106 and B9 were micropropagated using a liquid culture system. Three different strengths of 0.8% agar solidified PGR free basal MS medium were first tested to optimize the culture media for both the rootstocks. Full strength medium (MS0) supported maximum in vitro growth, multiplication, rooting and survival under field conditions as oppos...

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l myo-inositol, 2 mg·l thiamineHCl, 1 mg·l piridoxine-HCl, 1 mg·l nicotinic acid and 30 g·l sucrose and 8 g·l agar was considered together with two concentrations of BA (0,2 and 2 mg...

Influence of fructose, sucrose, glucose, sorbitol and maltose carbon sources at various concentrations on rooting and hyperhydricity apple rootstock MM106 was investigated. Type and concentration of sugars had a significant effect on rooting percentage, mean root number, mean root length, hyperhydricity, as well as survival rate. The in vitro cultures produced the higher rooting percentage, mea...

the purpose of this study was to compare rooting methods in tissue culture regenerated-clones in sugar beet and their adaptation to environmental conditions and determine clones ploidy stability. two kinds of explants including shoot apex of seedlings and inflorescence stem pieces were used for preparation and propagation of the clones in media. in the next step, three rooting and adaptation me...

This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary no...

Rosa persica with a distinctive red eye is a good candidate for addition to the breeding pool of roses. In order to optimize micropropagation of R. persica, three culture media (MS, QL and VS) were compared. The maximum growth rate was observed in the VS medium. Addition of FeEDTA (Fe: 65.4 mg/l), calcium gluconate (Cg: 2.7 g/l) and Gamborg vitamins (B5) instead of VS vitamins, alone or in comb...

• Tree root respiration utilizes a major portion of the primary production in forests and is an important process in the global carbon cycle. Because of the lack of ecologically relevant methods, tree root respiration in situ is much less studied compared with above-ground processes such as photosynthesis and leaf respiration. • This study introduces a new 13 C natural tracer method for measuri...