This is a short review on the isolation and translation in heterologous Systems of a 9 s RNA which carries the information of haemoglobin. Rabbit haemodobin (Hb) messenger RNA (mRNA) was the first eukaryotic genetic message to be isolated, quite a long time ago (1). This was mainly due to the fact that mammalian reticulocytes offer the favourable opportunity of being highly specialized cells wh...

Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenger RNA directs the initiation, synthesis, and release of a product that was ide...

The Kleinschmidt technique has been used to observe reovirus cores that have synthesized messenger RNA. Some individual viral cores probably synthesize all ten messenger RNA molecules in vitro. Each messenger RNA molecule appears to be attached to a different site on the core surface, implying that there are probably a number of different enzymic sites in each core.

An intracellular assay for viral envelope glycoprotein (env) messenger was employed to analyze the RNA from virus particles of Rous-associated virus type 2. For this assay RNA was microinjected into cells infected by the env-deficient Bryan strain of Rous sarcoma virus [RSV(-) cells]. Only when the injected RNA could be translated by the recipient cells to produce viral envelope glycoprotein wa...

Comprehensive identification of potential immune-related genes related to tumor biological behavior and their significance in the treatment head neck squamous cell carcinoma with a messenger RNA (mRNA) vaccine