نتایج جستجو برای: rna isolation

تعداد نتایج: 376688  

Journal: :Nucleic Acids Symposium Series 2005

Journal: :Journal of clinical microbiology 2005
Francescopaolo Di Cello Yi Xie Maneesh Paul-Satyaseela Kwang Sik Kim

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.

2017
Yue-Ting Tang Yi-Yao Huang Lei Zheng Si-Hua Qin Xu-Ping Xu Tai-Xue An Yong Xu Ying-Song Wu Xiu-Mei Hu Bao-Hong Ping Qian Wang

Exosomes are cell-derived vesicles and are abundant in biological fluids; they contain RNA molecules which may serve as potential diagnostic biomarkers in 'precision medicine'. To promote the clinical application of exosomal RNA (exoRNA), many isolation methods must be compared and validated. Exosomes in cell culture medium (CCM) and serum may be isolated using ultracentrifugation (UC), ExoQuic...

2001
Donald A. Hunter Michael S. Reid

A simple RNA isolation method based on hot borate and proteinase K was used to extract high-quality RNA from petals of daffodils (Narcissus pseudonarcissus L. 'Dutch Master'). The method was adapted from the isolation technique described by Wan and Wilkins (1994). A single isopropanol precipitation and ethanol wash replaced all the second day steps of the Wan and Wilkins procedure. The RNA isol...

Journal: :Cytometry. Part A : the journal of the International Society for Analytical Cytology 2007
Elisabeth Mack Andreas Neubauer Cornelia Brendel

BACKGROUND RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. METHODS Small populations of K562 cells or PMBC were sorted into the lysing reagent and subjected to RNA extraction, employing eith...

2010
W. Walter Lorenz Yuan-Sheng Yu Jeffrey F. D. Dean

Tissues isolated from conifer species, particularly those belonging to the Pinaceae family, such as loblolly pine (Pinus taeda L.), contain high concentrations of phenolic compounds and polysaccharides that interfere with RNA purification. Isolation of high-quality RNA from these species requires rigorous tissue collection procedures in the field and the employment of an RNA isolation protocol ...

2014
Kunxi Ouyang Juncheng Li Hao Huang Qingmin Que Pei Li Xiaoyang Chen

Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, Neolamarckia cadamba contains large quantities of other components, particularly RNA-binding alkaloids, which makes the isolation even more challenging. Here, we describe a concise and efficient RNA isolation method that combines ...

ژورنال: علمی شیلات ایران 2014
علیشاهی, مجتبی, قاسمی, محدث, سیدمرتضایی, سیدرضا, صیفی آبادشاپوری, مسعودرضا,

ویرمی بهاره کپور (SVC)، توسط ویروسی باRNA تک رشته ای منفی از خانواده رابدوویریده ایجاد می شود. ویروس SVCV عامل اصلی بیماری مسری ویرمی بهاره کپور در سرتاسر جهان بخصوص در ماهی کپور معمولی پراکنده است و در دیگر خانواده های ماهیان شامل اردک ماهیان ، کپور ماهیان دندانه دار ، خورشید ماهیان ، گربه ماهیان و آزاد ماهیان نیز سبب بیماری می گردد. در این مطالعه به منظور دستیابی به روشی مناسب برای استخر...

Journal: :Analytical biochemistry 1987
C Puissant L M Houdebine

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

ژورنال: :علمی شیلات ایران 0
سیدرضا سیدمرتضایی s.r.s. mortezaei مجتبی علیشاهی m. alishahi مسعودرضا صیفی آبادشاپوری m. r. seifi محدث قاسمی m. qhasemi

ویرمی بهاره کپور (svc)، توسط ویروسی باrna تک رشته ای منفی از خانواده رابدوویریده ایجاد می شود. ویروس svcv عامل اصلی بیماری مسری ویرمی بهاره کپور در سرتاسر جهان بخصوص در ماهی کپور معمولی پراکنده است و در دیگر خانواده های ماهیان شامل اردک ماهیان ، کپور ماهیان دندانه دار ، خورشید ماهیان ، گربه ماهیان و آزاد ماهیان نیز سبب بیماری می گردد. در این مطالعه به منظور دستیابی به روشی مناسب برای استخراج rn...

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