RNA interference (RNAi) is a biological mechanism that involves the inhibition of gene expression by introduction double-stranded (dsRNA) molecules are complementary to specific target genes. This technique has emerged as promising strategy for management insect pests in crops. The application RNAi pest use dsRNA essential genes species. When these ingested pests, they trigger silencing inhibit...

RNAi (RNA interference) refers to the suppression of expression of a target gene (mainly at the post-transcriptional or translational level) induced by small (21-23 nucleotides) RNA molecules, including siRNA (small interfering RNA). Suppression of gene expression by RNAi represents an important part of the regulation of gene expression. Interestingly, recent advancements in RNAi research suppo...

RNA interference (RNAi) is a simple and rapid method for silencing genes in various organisms, it widely used gene function genetics research. RNAi has been developed as novel pest management strategy. Double-stranded RNAs (dsRNAs) delivered via microinjection, ingestion, or soaking are effective insect pests, whereas oral topical delivery methods feasible field applications. Here, we summarize...

Crop plants suffer severe yield losses due to the significant damages caused by aphids. RNA interference (RNAi) technology is a versatile and environmentally friendly method for pest management in crop protection. Transgenic expressing siRNA/dsRNA non-transformative methods such as spraying, microinjection, feeding, nanocarrier-delivery-mediated RNAi approach have been successfully applied agri...

RNA interference (RNAi)-based pesticides are pest control agents that use RNAi mechanisms as the basis of their action. They regarded environmentally friendly and a promising alternative to conventional chemical pesticides. The effective substance in RNAi-based is double-stranded (dsRNA) designed match nucleotide sequence target essential gene concern. When taken up by pest, this exerts an effe...

Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread ge...

RNA interference (RNAi) is a broadly used reverse genetics method in C. elegans. Unfortunately, RNAi does not inhibit all genes. We show that loss of function of a putative RNA-directed RNA polymerase (RdRP) of C. elegans, RRF-3, results in a substantial enhancement of sensitivity to RNAi in diverse tissues. This is particularly striking in the nervous system; neurons that are generally refract...