The heating of microfluidic channels such as occurs in cell stretching and sorting devices was examined using the established technique of recording the intensity ratio between two fluorescent dyes, Rhodamine B (RhB) which is temperature sensitive and Rhodamine 110 (Rh110) which is not[1]. Microfluidic devices each consisting of a single channel and waveguide input were manufactured using ultra...

Photoactivatable “caged” fluorophores enable numerous advanced biological imaging experiments, including photoactivated localization microscopy (PALM) and related super-resolution imaging techniques. Of the extant fluorophore scaffolds, caged rhodamines and fluoresceins display properties that are exceptionally well suited for superresolution microscopy, exhibiting high contrast and photon yiel...

We report on strong coupling between surface-plasmon polaritons (SPP) and Rhodamine 6G (R6G) molecules, with double vacuum Rabi splitting energies up to 230 and 110 meV. In addition, we demonstrate the emission of all three energy branches of the strongly coupled SPP-exciton hybrid system, revealing features of system dynamics that are not visible in conventional reflectometry. Finally, in anal...

The passive mode locking of an argon-ion-pumped energy-transfer cw dye laser operating in the near infrared is reported. The dyes 1,1'-diethyl-4,4'-carbocyanine iodide and 1,1'-diethyl-2,2' -dicarbocyanine iodide were used separately to mode lock a gain medium comprising the dyes DCM and Rhodamine 700. Subpicosecond pulses were generated from 732 to 761 nm in a simple linear cavity, and pulses ...

We investigated the impact of crystallographic orientation on the photocatalytic activity of single crystalline WO3 thin films prepared by molecular beam epitaxy on the photodegradation of rhodamine B (RhB). A clear effect is observed, with (111) being the most reactive surface, followed by (110) and (001). Photoreactivity is directly correlated with the surface free energy determined by densit...

Caspases (cysteine aspartate-specific proteases) are a structurally related group of cysteine proteases that cleave peptide bonds following specific recognition sequences. They play a central role in activating apoptosis of vertebrate cells. To measure apoptosis induced by various stimuli and at an early apoptotic stage, caspases are an ideal target. This is especially the case when apoptotic c...

A fluorescence correlation spectroscopy experiment that combines two-photon excitation and a standing-wave interference pattern is presented. The experimental correlation function can be analyzed using a simple expression involving (1) an exponential decay with time constant tau(f), which reflects diffusion across the interference fringes, and (2) a longer-lived decay with time constant tau(ome...

Fluorescent molecules are essential for basic research in the biological sciences and have numerous practical applications. Herein is described the synthesis and use of a new class of latent fluorophores based on a novel design element, the trimethyl lock, that confers distinct advantages over extant fluorophores and pro-fluorophores. A diacetyl version of the latent fluorophore is stable in a ...

Rice is the second most extensively consumed food ingredient, and its by-products in paddy field include rice husk straw. ash, resulting from burning, considered an environment menace, inducing negative effects on area which it disposed of. In this study, was applied as a silicate source to obtain mesoporous silica material. Characterization techniques confirmed well-ordered mesophase resemblan...