When adult males of the smaller brown planthopper, Laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. Further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. When the butanol fr. was charged on an ODS open column and el...

Wolbachia species are intracellular symbionts that cause reproductive alterations in arthropods. Transinfection experiments have been performed in many arthropod species to elucidate the interaction betweenWolbachia and a new host. To ease transinfection of this bacterium to new arthropodhosts,we introduced two techniques: nymphal injection insteadof embryonic injection and the use of a culture...

The rice planthoppers, Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus, harbor intracellular yeast-like symbiotes (YLS) in the fat body. The YLS are obligated endosymbiotes and are transovarially inherited. Nucleotide sequences of the 18S rRNA genes from the YLS were analyzed by direct sequencing. The YLS of the three species of planthoppers were monophyletic and their taxon...

Persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. However, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. In this study, we used Rice stripe virus (RSV), its insect vector Laodelphax striatellus and host plant (Oryza sativa) to explore how persist...

The flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five Hemipterans, and two hymenopterans. Among which, the coleopteran Lissorhoptrus oryzophilus (Kuschel) had the largest genome of 981 Mb. The average genome size was 504 Mb, suggesting that insects have a moderate-size genome. Compared with the insects in other...

The spatial distribution of crop and non-crop habitats over segmented agricultural landscapes could be used as a means to reduce insect pest populations. Seven land cover categories such as wheat, rapeseed, vegetable, water, built-up, paved road, and unsurfaced road were extracted from GeoEye satellite images dating from late May to late June of 2010. Three diversity metrics and three evenness ...

Cytochrome P450 monooxygenases (CYPs) usually overexpressed in resistant strain were found involved in oxidative detoxification of insecticides. In this study, an investigation was conducted to confirm if resistance irrelevant CYPs which were not overexpressed in resistant strain before, were capable of degrading insecticides. Three resistance irrelevant CYPs viz. CYP417A2v2, CYP425A1v2, and CY...

Most plant viruses are transmitted by hemipteroid insects. Some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. Rice stripe virus (RSV), a Tenuivirus, transmitted mainly by the small brown planthopper (Laodelphax striatellus), is also spread to the offspring through the eggs. Here, we used the ...

CYP353D1v2 is a cytochrome P450 related to imidacloprid resistance in Laodelphax striatellus. This work was conducted to examine the ability of CYP353D1v2 to metabolize other insecticides. Carbon monoxide difference spectra analysis indicates that CYP353D1v2 was successfully expressed in insect cell Sf9. The catalytic activity of CYP353D1v2 relating to degrading buprofezin, chlorpyrifos, and de...