The objective of the present study was to develop a simple, rapid and sensitive reversedphase high performance liquid chromatographic (RP-HPLC) analytical method with UV detection system for the quantitative determination of metronidazole in human plasma. The chromatographic separation was performed by using C18 RP column (250mm X 4.6mm, 5μm) as stationary phase and 0.01M potassium dihydrogen p...

INTRODUCTION Chromatography is probably the most powerful analytical technique available to the modern chemist. Its power arises from its capacity to determine quantitatively many individual components present in mixture by single analytical procedure[1-2]. High-performance liquid chromatography (HPLC) is a chromatographic technique that can separate a mixture of compounds and is used in bioche...

A new conjugate, 1 -(y-i-glutamylamino)cyclopropane-1 -carboxylic acid (CACC), of the ethylene precursor 1 -aminocyclopropane1 -carboxylic acid (ACC) i s identified. The only previously identified conjugate of ACC i s 1 -(malonylamino)cyclopropane-1 -carboxylic acid (MACC). CACC, not MACC, was the major conjugate formed by crude protein extracts of tomato (Lycopersicon esculentum Mil1 cv Ailsa ...

University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530 003, Andhra Pradesh, India ABSTRACT: A simple, rapid, precise and accurate isocratic reversedphase stability-indicating HPLC method was developed and validated for the simultaneous determination of Atazanavir (AT) and Ritonavir (RT) in commercial tablets. The method has shown adequate separation for AT, RT from ...

Chborhexidine, a potent antiseptic (1), has been used as a preservative for urine (2, 3) in which oxabic acid and calcium were to be measured. We recently described a biquid-chromatographic method for determining oxalic acid in urine (4) and wish to describe our results when using chlorhexidine gluconate (CG) to replace HCI in the preservation of urine in which oxalic acid was measured. Our met...

Systemic Lupus Erythematosus (SLE) is an autoimmune disease with chronic inflammation affecting multiple organ systems, as well as accelerated atherosclerosis as a major complication. Prior studies by our lab have shown beneficial effects of PPARγ agonists towards preventing SLE in two different mouse models: the well-established lupus mouse model, MRL.lpr, and the gld.apoE -/model of accelerat...

Chronic pancreatitis (CP) is currently diagnosed using invasive endoscopic and imaging techniques. However, urine can be collected safely and noninvasively and as such may offer a superior alternative to current techniques of CP diagnosis. We use mass spectrometry-based methods to discover proteins which are exclusive to or differentially abundant in urine of chronic pancreatitis patients. We h...

In the study of fat metabolism, it is important to examine the behaviour of each constituent fatty acid of the ingested fat under investigation. For this purpose a method is necessary by which a quantitative determination of each of the different saturated and unsaturated fatty acids can be carried out, not only in the fat ingested but also in the fat of the blood, chyle and faeces. Reversedpha...

Estimation of related substancesby usinghigh-performance liquid chromatographic method was developed and validated for the determination of Tigecycline in the present work. Reversedphase chromatography was performed on Waters 2489 UV 2695 pump, Waters 2998 PDA 2695 pump Software Empower2 photodiode array detector using Zorbax Eclipse plus C18 (100 mm × 4.6 mm, 1.8 μm particle size) column with ...

Studies on translation frequently require large amounts of purified individual tRNAs. However, few purified tRNAs are commercially available. Individual tRNAs can be easily produced by in vitro transcription, but the lack of modifications of the tRNA transcripts may influence or impair their function [1,2]. The purification of specific tRNAs from total tRNA is a laborious process that requires ...