Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction [Review]. Eur J Clin Chem Clin Biochem 1997;35:3–10. 14. Bostick PJ, Chatterjee S, Chi DD, Huynh KT, Giuliano AE, Cote R, Hoon DS. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the ly...

background and aims: the causal agents of viral lettuce big vein disease are two viruse, lettuce big vein associated virus (varicosavirus) and mirafiori lettuce virus (ophiovirus). these viruses have coat proteins of similar size but have different morphologies and serologically unrelated.the purpose of this study was to distinguish and detect lbvav and milv in lettuce fields in tehran province...

The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform. These characteristics have made it the method of choice for minimal residual disease monitoring suc...

BACKGROUND Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination. RESULTS The use of a relational database, in conjunction with a series of scripts written i...

A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecti...

Knowing the underlying photophysics of thermally activated delayed fluorescence (TADF) allows proper design of high efficiency organic light-emitting diodes. We have proposed a model to describe reverse intersystem crossing (rISC) in donor-acceptor charge transfer molecules, where spin-orbit coupling between singlet and triplet states is mediated by one of the local triplet states of the donor ...

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

A variety of chemically synthesized oligonucleotides designed on the basis of amino acid and/or nucleotide sequence data were used to detect a large number of novel reverse transcriptase coding sequences in human and mouse DNAs. Procedures involving Southern blotting, library screening, and the polymerase chain reaction were all used to detect such sequences; the polymerase chain reaction was t...

Abbreviations: ARP: acidic ribosomal protein CHS: chalcone synthase DDRT-PCR: differential display reverse transcriptase polymerase chain reaction DETs: differentially expressed transcripts DPA: days post anthesis ESTs: expressed sequence tags MBD: methyl CpG binding domain OPT: oligopeptide transporter protein PCR: polymerase chain reaction PG: polygalacturonase enzyme RRF: ribosome recycling ...