Two UV-spectrophotometric and one reverse phase high performance liquid chromatography methods have been developed for the simultaneous estimation of amlodipine besilate, losartan potassium and hydrochlorothiazide in tablet dosage form. The first UV spectrophotometric method was a determination using the simultaneous equation method at 236.5, 254 and 271 nm over the concentration range 5-25, 10...

A novel, efficient and simple approach for soy phosphatidylcholine analysis according to its fatty acid composition was studied with reverse-phase high-performance liquid chromatography. The reverse-phase high-performance liquid chromatography analysis was performed isocratically using UV detector and simple mobile phase solvents consisting of isopropyl alcohol, methanol, and deionized water in...

Avian luteinizing hormone-releasing hormone (LHRH) has been isolated from 249,000 chicken hypothalami and shown to differ structurally from mammalian hypothalamic LH-RH. Purification was achieved by acetic acid extraction, anti-LH-RH affinity chromatography, and cation exchange and reverse phase high performance liquid chromatography. The isolated peptide eluted as a single peak on reverse phas...

304 CLINICALCHEMISTRY,Vol.38, No.2, 1992 chain length on protein retention, resolution, and recovery implications for preparative HPLC. J Liq Chromatogr 1983;6:497-510. 4. Tarr GE, Crabb JW. Reverse-phase high-performance liquid chromatography of hydrophobic proteins and fragments thereof. Anal Biochem 1983;131:99-107. 5. Wu D, Walters ER. Stationary-phase ligand density effects in reversed-pha...