According to the reports of WHO, stability of Rev.1 vaccine should take more than one year, while the expiring date for the vaccine produced in Iran is 3 to 4 months, therefore any attempt to elongate the stability of this vaccine can solve many problems of the production including the request of Veterinary Organization of Iran in this regard. The objective of this study was to increase the sta...

The aim of this study was to prepare liposomes of acyclovir (ACY) by thin layer evaporation (TLE) and reverse phase evaporation (REV) methods. Twenty-seven batches of liposomes from each method were prepared using technique of three variables at three levels (33) factorial design. Drug/Lipid (molar ratio), hydration volume and hydration time were considered three independent variables in TLE me...

The human immunodeficiency virus type 1 (HIV-1) Rev and human T-cell leukemia virus type 1 (HTLV-1) Rex proteins are essential for the expression of viral structural proteins and productive infection. Both contain a nuclear export signal (NES) in their C-terminal domain and a nuclear localization signal (NLS) in their N-terminal domain. The NES and NLS are necessary for shuttling between nucleu...

The aim of this study was to prepare liposomes of acyclovir (ACY) by thin layer evaporation (TLE) and reverse phase evaporation (REV) methods. Twenty-seven batches of liposomes from each method were prepared using technique of three variables at three levels (33) factorial design. Drug/Lipid (molar ratio), hydration volume and hydration time were considered three independent variables in TLE me...

The HIV-1 REV protein binds to the stem II region of the REV-responsive element (RNA). Studies to further define the RNA sequence and structure specifically bound by REV protein identify a minimal RNA element of 40 nucleotides. Analysis of RNA fragments by gel retardation and filter binding suggest that a core element composed of one particular stem with flanking sequences capable of forming a ...

The use of RNA transport elements from different viruses can provide novel attributes to HIV-1-based gene delivery systems such as improved safety or Rev independence. We previously described an HIV-1 based gene delivery system that utilized the simian immunodeficiency virus Rev-response element (RRE) in place of the HIV-1 RRE. Despite the use of Rev for the production of vector stocks, we show...

Human immunodeficiency virus type 1 (HIV-1) gene expression in astrocytes is restricted, resulting in a brief and limited synthesis of HIV-1 viral structural proteins. Impaired Rev function has been documented in these cells. However, the molecular mechanisms underlying the impaired Rev function are not fully understood. Using the astroglial cell line U87.MG as a model, we report here that HIV-...

Expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA. Furthermore, the interaction of the Rev activation domain with a cellular cofactor is essential for Rev function in vivo. Using cross-linking e...

The controversy about a Cambrian “explosion” of morphological disparity (followed by decimation), cladogenesis and fossilization is of central importance for the history of life. This paper revisits the controversy (with emphasis in onychophorans, which include emblematic organisms such as Hallucigenia), presents new data about the Chengjiang (Cambrian of China) faunal community and compares ii...